小分子糖类负载后血小板超微结构和功能初步研究  

Ultrastructural and Functional Assessment on Platelets Loaded with Small Molecule Carbohydrates

作　　者：杨超[1] 王捷熙[1] 韩颖[1] 王艳[1] 权国波[1] 刘敏霞 高峰[1] 刘安[1] 

机构地区：[1]军事医学科学院输血医学研究所,北京100850

出　　处：《中国实验血液学杂志》2008年第3期687-690,共4页Journal of Experimental Hematology

摘　　要：本研究探讨血小板在37℃条件下负载小分子糖类物质4小时后形态、结构和功能的变化。应用透射电子显微镜观察小分子糖类物质负载前后血小板超微结构的变化,用血细胞计数仪检测血小板数及血小板平均体积(MPV)的变化,用血小板聚集测试仪检测血小板最大聚集率,采用流式细胞术检测血小板膜表面标志CD62p及磷脂酰丝氨酸(PS)。结果表明:小分子糖类物质负载后,血小板超微结构无明显变化;血小板最大聚集率可达新鲜血小板的60%以上;血小板计数和血小板平均体积(MPV)与新鲜血小板相比无显著差异(p>0.01);血小板膜表面标志CD62p的标记率及Annexin V的结合率与新鲜血小板相比均无明显差异。结论:小分子糖类物质负载后血小板仍然具有相对完整的超微结构和较好的聚集活性,功能基本正常。The aim of this study was to investigate the effect of loading some small molecule carbohydrates into human platelets on ultrastucture and function. The ultrastructure of platelets were observed by transmission electron microscope （TEM）; the platelet counts and mean platelet volume （MPV） were measured by hemocytometer, the maximal platelet aggregation rate was measured optically in an aggregometer; the surface marker of platelet membranes CD62p and phosphatidyl serine were analyzed by flow cytometry. The results showed that no significicant changes of the ultrastructure of platelets loaded with small molecule carbohydrates were seen. The aggregation responsiveness of platelets loaded with small molecule carbohydrates reached to 60% of the fresh control platelets. The values of platelet counts and MPV showed no significant differences. The expression level of CD62p and the binding rate with Annexin V before and after loading small molecule carbohydrates into platelets were no different. It is concluded that the platelets after loading with small molecule carbohydrates remained fine ultrastructure and function.

关 键 词：小分子糖类物质 血小板 超微结构 功能评价 

分 类 号：R331.143[医药卫生—人体生理学]