单宁酸铽离子配合物与伴清蛋白相互作用  

Interaction of the Complexes of Tannic Acid with Terbium(Ⅲ) to Apoovotransferrin

作　　者：李晓莉[1] 赵春贵[1] 

机构地区：[1]山西大学化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《山西大学学报（自然科学版）》2008年第1期92-94,共3页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(30740038);山西省自然科学基金(20031017)

摘　　要：在室温条件下,pH 7.4、0.01 mol.L-1Hepes缓冲液中,用荧光光谱法研究了CTb/CTA摩尔比分别为1∶1、2∶1和4∶1的单宁酸与铽离子的复合物与伴清蛋白(apoOTF)的相互作用.研究表明:单宁酸与Tb3+结合形成1∶2配合物,其结合能力明显大于apoOTF与Tb3+的结合能力.配合物对apoOTF的荧光有强烈的猝灭作用,其作用机理属静态猝灭.At room-temperature,when the molar ratio of CTb/CTA were 1 ： 1,2 ： 1 and 4 ： 1,the interaction of the complexes of tannic acid（TA） with Terbium（ Ⅲ ） to apoovotransferrin （apoOTF） was investigated by fluorescence spectroscopy at pH7.4,0. 01 mol · L^-1 Hepes. The results showned that TA and Tb^3＋ formed 1：2 complexes which constant KA was bigger than that of apoOTF and Tb^3＋. The complex of TA- Tb2^3＋ had powerful ability to quench the fluorescence of apoOTF and the type of quenching was the static quench. Between TA-Tb2^3＋ and the protein formed 1 ： 1 complex.

关 键 词：伴清蛋白 单宁酸 铽 荧光光谱 

分 类 号：Q657[生物学—生物物理学]