不同VNTR位点组合用于北京基因型结核分枝杆菌基因分型的研究  

Genotyping of Beijing genotype strains isolated from Beijing area by different combinations of variable-number tandem-repeat loci

作　　者：李墨[1] 焦伟伟[1] 孙桂芝[2] Igor Mokrousov 郭雅洁[1] 申阿东[1] 

机构地区：[1]首都医科大学附属北京儿童医院儿科研究所,北京100045 [2]北京胸科医院检验科,北京100095 [3]俄罗斯圣彼得堡巴斯德研究所,圣彼得堡197101

出　　处：《中国人兽共患病学报》2008年第6期505-509,共5页Chinese Journal of Zoonoses

基　　金：国家自然基金资助项目(30471841)

摘　　要：目的评价不同串联重复序列(VNTR)位点组合在北京地区北京基因型结核分枝杆菌基因分型中的应用。方法采用多位点数目可变串联重复序列分析(MLVA)技术对72株北京地区北京基因型菌株的24个VNTR位点进行分析,基因多态性分析采用BioNumerics软件。结果24个位点的多态性差异较大,位点QUB-11b(HGI 0.651)、Mtub 21(HGI0.556)和QUB-26(HGI 0.518)的多态性较高,有两个位点(MIRU 2和MIRU 24)则不具有多态性;不同VNTR位点组合(12位点,15位点,24位点)在北京基因型菌株中的分辨指数依次升高,分别为0.788,0.990,0.992,后两个组合的差异仅是由位点Mtub 29引起的。结论不同的VNTR位点在北京地区北京基因型菌株中的分辨力存在差异;推荐的基础VNTR 15位点与Mtub 29组合可作为北京地区结核分枝杆菌分子流行病学研究的一线方法。The newly proposed variable-number tandem-repeat （VNTR） typing system, including basic 15-locus set and high-resolution 24-locus set, were evaluated in 72 Beijing genotype Mycobacterium tuberculosis isolated from Beijing area in China. As a result, 24 loci showed different discriminatory, in which QUB-11b （HGI 0. 651）, Mtub 21 （HGI 0. 556） and QUB-26 （HGI 0. 518） were the relatively more polymorphic loci, MIRU 2 and MIRU 24 had extremely low resolution among Beijing genotype strains （HGI 0）. The most widely used 12-locus set had limited power （HGI 0. 788） in this sample collection. A dramatically increased resolution was observed by using the proposed VNTR 15-locus and 24-locus set, with HGI of 0. 990 and 0. 992, respectively. The subtle difference in HGI was originated from one locus Mtub 29, which was included in 24-locus system. Thus, the proposed VNTR 15-locus plus Mtub 29 can be served as the first screening method in routine epidemiological study in Beijing area.

关 键 词：结核分枝杆菌 北京基因型 串联重复序列 基因分型 

分 类 号：R521[医药卫生—内科学]