肿瘤坏死因子超家族成员4基因SNP rs3861950与脑梗死  

The study on the association between the SNIP rs3861950 of tumor necrosis factor ligand superfamily member 4 gene and cerebral infarction

作　　者：黄清[1] 杨期东[1] 刘运海[1] 夏琨[2] 朱赞华[2] 葛亮[3] 刘宁[1] 谭若利[1] 唐建生[1] 王玮[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008 [2]中南大学医学遗传学国家重点实验室 [3]湖南省人民医院神经内科

出　　处：《中华神经科杂志》2008年第6期371-375,共5页Chinese Journal of Neurology

摘　　要：目的探讨肿瘤坏死因子超家族成员4（TNFSF4）基因SNPrs3861950与本地区脑梗死发病的关系。方法采用TaqMan-PCR方法检测TNFSF4基因SNPrs3861950基因型与等位基因频率。湖南籍汉族脑梗死共287例，对照组为湖南籍汉族健康体检者共285名。结果脑梗死组CC基因型（7．7％）分布与对照组（2．1％）相比，差异具有统计学意义（r=9．553，P=0．008）；C等位基因频率脑梗死组（0．190）高于对照组（0．137，x^2=5．887，P=0．015）。动脉血栓性脑梗死亚组与对照组比较其基因型（r=9．396，P=0．009）及等位基因频率（x^2=6．134，P=0．013）差异均有统计学意义。非条件Logistic多因素回归分析显示CC基因型使脑梗死发病的风险是其他危险因素的3．7倍（P=0．002，OR3．706）。结论TNFSF4基因rs3861950C→T与湖南籍汉族脑梗死发病相关，尤其与动脉血栓性脑梗死发病相关，rs3861950C等位基因可能是湖南籍汉族脑梗死发病的独立危险因素。Objective To investigate the association of the tumor necrosis factor ligand superfamily member （TNFSF）4 gene polymorphisms rs3861950 and cerebral infarction in Hunan area. Methods The frequencies of the genotypes of rs3861950 were detected utilizing real-time fluorescent PCR method based on TaqMan probe. Subjects examined were composed of 287 patients and 285 healthy individuals. Results There were significant differences in TNFSF4 gene rs3861950 C→T site polymorphism and allele frequency between the subjects and the controls, the distribution of CC genotype was significantly higher in the former （7.7%） than in the later （2. 1%, X2 = 9. 553, P = 0. 008 ）, so was the frequencies of C allele （ 0. 190 vs 0. 137, X2 = 5. 887 ,P = 0. 015 ）. Moreover, there were significant differences in the distribution of genotype and the frequencies of allele between the subgroup of cerebral thrombosis and control group （for the distribution of genotype X2 = 9. 396 P = 0. 009, for the frequencies of allele X2 = 6. 134, P = 0. 013 ）. Logistic regression analysis showed that the risk factor of CC genotype was 3.7 times higher than others （ P = O. 002, OR 3.706）. Conclusions TNFSF4 gene SNP rs3861950 is associated with cerebral infarction and C allele is considered to be one of independent risk factors in the Han population in Hunan Province.

关 键 词：脑梗塞 膜糖蛋白类 肿瘤坏死因子类 多态性 单核苷酸 聚合酶链反应 

分 类 号：R686[医药卫生—骨科学]