牙科季铵盐类单体的制备及其抗菌作用的研究  

Preparation and antibacterial activity of quaternary ammonium salt monomers

作　　者：肖玉鸿[1] 陈吉华[1] 方明[1] 邢晓东[2] 李芳[1] 柴治国[1] 

机构地区：[1]第四军医大学口腔医学院修复科,西安710032 [2]清华大学化工系高分子研究所

出　　处：《中华口腔医学杂志》2008年第6期370-373,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30772444）

摘　　要：目的制备3种季铵盐类单体，比较其对口腔常见细菌的作用，为牙科抗菌材料的研制提供基础依据。方法制备3种季铵盐类单体[甲基丙烯酰氧乙基．苄基-二甲基氯化铵（ methacryloxylethyl benzyl dimethyl ammonium chloride, DMAE-BC）、甲基丙烯酰氧乙基-间氯苄基-二甲基氯化铵（methacryloxylethyl m-chloro benzyl dimethyl ammonium chloride, DMAE-m-CBC）、甲基丙烯酰氧乙基-正十六烷基-二甲基氯化铵（methacryloxylethyl cetyl dimethyl ammonium chloride, DMAE-CB）]，采用液体稀释法比较其对变形链球菌、血链球菌、牙龈卟啉单胞菌、产黑普氏菌的抗菌作用。结果3种季铵盐类单体中DMAE-CB的抗菌效果最强，对4种口腔细菌的最低抑菌浓度值为1．2至4．8ms／L，而另外两种单体对4种细菌的最低抑菌浓度值为1562．5至3125．0mg／L。结论3种季铵盐类单体对变形链球菌、血链球菌、牙龈卟啉单胞菌、产黑普氏菌均有不同程度的抗菌作用，其中DMAE-CB的抗菌活性最强。Objective To prepare three quaternary ammonium salt （QAS） monomers, and to compare their antibacterial activities against four oral bacterial strains. Methods Three antibacterial monomers[methacryloxylethyl benzyl dimethyl ammonium chloride（ DMAE-BC ）, methacryloxylethyl m-chloro benzyl dimethyl ammonium chloride（ DMAE-m-CBC）, methacryloxylethyl cetyl dimethyl ammonium chloride （DMAE-CB） ] were synthesized according to the general structure of target monomers. Their antibacterial effects were investigated using the broth dilution test on Gram-positive and Gram-negative bacterial strains （ Streptococcus mutans,Streptococcus sanguis, Porphyromonas gingivalis, Prevotella melaninogenica）. Results Three different monomers were successfully obtained. All the tested bacterial strains were susceptible to the three monomers, among which DMAE-CB exhibited the lowest minimal inhibitory concentrations （ MIC ） ranging from 1.2 to 4. 8 mg/L. Conclusions All these three QAS monomers have different antibacterial activities against four oral bacteria strains. The data indicate that DMAE-CB may be a candidate antibacterial agent for oral infectious diseases.

关 键 词：微生物敏感性试验 牙科材料 季铵盐 单体 

分 类 号：R686[医药卫生—骨科学]