丝状真菌蛋白质组分析  

Proteome analysis of animal filamentous fungi

作　　者：李锐[1] 贺亮[1] 邓旭明[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062

出　　处：《东北农业大学学报》2008年第5期58-61,共4页Journal of Northeast Agricultural University

基　　金：国家高新技术研究和发展资助项目(2006AA020504)

摘　　要：试验建立液氮冷冻研磨、蜗牛酶消化破壁、尿素裂解、同时结合硫酸铵沉淀预分离的方法研究丝状真菌蛋白质组,得到的肽片段混合物通过二维液相色谱分离检测,进行蛋白质组分析。本方法提高了蛋白的分离效率和提取数量,特别是对于那些功能重要但丰度低的蛋白,适合于丝状真菌蛋白组学研究中目的蛋白的提取。试验共鉴定了396个蛋白,包括了参与各种细胞生理过程的蛋白,为进一步开展动物丝状真菌蛋白质组学研究打下了良好的基础。Liquid nitrogen grinding, snailase digestion, urea lysis and ammonium sulfate precipitation methods was established for filamentous fungal proteome analysis. The recovered peptide mixture was further separated through strong cation exchange column, then ionized and detected with ESI-MS/MS. This method improved the efficiency of protein extraction, especially for proteins that are low abundance but have important function. Total 396 T. rubrum proteins were unambiguously identified involving in major biological processes and provided a basid for further fungal proteomic research.

关 键 词：丝状真菌 尿素裂解 蛋白质组 

分 类 号：Q949.32[生物学—植物学] Q51