X连锁凋亡抑制蛋白在胆脂瘤中的表达及其与细胞凋亡的关系  

The expression of XIAP in middle ear Cholesteatoma and the relationship with apoptosis

作　　者：关芳灵[1] 马敏[1] 尚丽芳[1] 白仙娥[1] 郝艳 

机构地区：[1]山西医科大学第二医院,030001 [2]长治和平医院

出　　处：《山西医药杂志（上半月）》2008年第6期492-494,共3页Shanxi Medical Journal

摘　　要：目的研究X连锁凋亡抑制蛋白(XIAP)在胆脂瘤中的表达及胆脂瘤中的凋亡情况,探讨两者之间的关系。方法①运用免疫组织化学两步法检测25例胆脂瘤标本及10例外耳道正常皮肤组织中XIAP的表达。②采用末端转移酶介导的原位缺口末端标记染色(TUNEL)技术检测两者组织的凋亡状态。结果①与正常上皮比较,胆脂瘤上皮中的XIAP蛋白表达明显下调(Z=-2.411,P<0.05)。②在胆脂瘤上皮中,细胞凋亡显著高于正常上皮组织(Z=-2.712,P<0.01)。③在胆脂瘤上皮中,XIAP的表达与凋亡呈负相关(rp=-0.527,P<0.01)。结论XIAP在胆脂瘤中的表达明显下调,与胆脂瘤上皮的凋亡密切相关,提示XIAP可能参与胆脂瘤上皮的凋亡调控过程,在胆脂瘤的发生、发展过程中起重要作用。Objective To establish the exprexsion of XIAP and the al2optosis status in middle ear cholesteatoma, to discuss the relationship between XIAP and apoptosis status in middle ear cholesteatoma. Methods （1）Specimens of 25 cholesteatoma and 10 normal skin collected from the external ear auditory canal were examined by immunohistochemical method using polyclonal antibodies of XIAP. （2）The apoptosis status of the 2 tissues were determined with TUNEL methods. Results （1）Comparing with normal external ear auditory canal skin, the expression of XIAP protein decreased obviously in cbolesleatoma （ Z = - 2.411, P 〈 0.05 ）. （2）The apoptosis of cholesteatoma tissue were significantly higher than that in normal external ear auditory canal skin （Z = - 2.712, P 〈 0.01 ）. （3）In cholesteatoma epithelium, the expression of XIAP was inversely correlated with apoptosis （rp = - 0.527, P 〈 0.01 ）. Conclusion The XIAP protein expressed less in cholesteatoma than normal meatal skin. There was a close correlation between the expression of XIAP protein and the cell apoptosis in cholesteatoma tissue. It suggests that XIAP participates in the regulation of apoptosis and plays important roles in the formation and development of cholesteatoma.

关 键 词：胆脂瘤 凋亡抑制蛋白 凋亡 

分 类 号：R764[医药卫生—耳鼻咽喉科]