免疫层析法测定动物源性食品中磺胺嘧啶残留  

Determination of sulfadiazine residue in food products of animal origin by immunochromatographic assay

作　　者：王喜亮[1] 金秀娥[2] 李奎[1] 熊宁[1] 石德时[1] 毕丁仁[1] 

机构地区：[1]华中农业大学动物医学院,武汉430070 [2]湖北省兽药监察所,武汉430064

出　　处：《中国抗生素杂志》2008年第6期350-354,共5页Chinese Journal of Antibiotics

基　　金：武汉市科技攻关计划项目(20022002062)

摘　　要：建立了动物源性食品中磺胺嘧啶残留的免疫层析快速测定方法。用胶体金标记磺胺嘧啶单克隆抗体作为显色剂，磺胺嘧啶竞争物包被于硝酸纤维素层析膜上作为捕获试剂，采用竞争反应模式制成胶体金免疫层析试纸。该免疫层析试纸可以在15min内完成对磺胺嘧啶残留的半定量检测。读条系统判定灵敏度为5ng／ml，肉眼判定检测限灵敏度为10ng／ml，与其它磺胺类药物无交叉反应。该方法在不同动物源性食品（鸡肉、鸡蛋、鸡肝、猪肉、猪肝、牛奶、蜂蜜）中添加磺胺嘧啶的回收率在68．1％-118．8％范围内。动物试验样品检测结果表明，该方法与ELISA及HPLC具有好的符合率。该方法灵敏度高，检测准确、简便、快速，有良好的开发应用前景。An immunochromatographic assay （ICA） was developed for the detection of sulfadiazine in food products of animal origin. Based on the competitive reaction mechanism, the competitor of sulfadiazine was coupled with the nitrocellulose membranes acted as the capture reagent. Colloidal gold was coulped with monoclonal antibody against sulfadiazine served as the detection reagent. With this method, semi-quantitative detection of sulfadiazine can be completed in less than 15min. The sensitivity to sulfadiazine was 5ng/ml by strip reader and 10ng/ml by eye measurement, and no cross-reactivity with other sulfonamides. The recoveries were from 68.1%-118.8% in different food products of animal origin （chicken muscle, egg, chicken liver, porcine muscle, porcine liver, milk, honey）. Results of animal experimental samples showed that there was a good agreement rate between ICA and ELISA as well as HPLC. This method is sensitive, accurate, simple, rapid and with a prominsing usage in practical application.

关 键 词：胶体金 免疫层析 磺胺嘧啶 

分 类 号：R978.1[医药卫生—药品]