ICP患者蜕膜NK细胞表型及杀伤活性的研究  

Study on the activity and phenotype of decidual natural killer cells in patients with Intrahepatic cholestasis of pregnancy

作　　者：肖敏[1] 凌斌[1] 周颖[2] 赵卫东[1] 冯定庆[2] 高宗侠[1] 程志祥[2] 

机构地区：[1]安徽医科大学附属省立医院妇产科,合肥230001 [2]安徽医科大学附属省立医院分子医学重点实验室,合肥230001

出　　处：《安徽医科大学学报》2008年第3期276-279,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科研基金资助项目(编号:34071805)

摘　　要：目的探讨妊娠期肝内胆汁淤积症(ICP)患者蜕膜自然杀伤(NK)细胞的数量、表型特征及杀伤活性,探讨其在ICP发病中的作用。方法选取确定ICP患者15例,正常晚期妊娠妇女15例,用流式细胞仪检测两组蜕膜组织中NK细胞数量及表型,用LDH法测定NK细胞杀伤活性。结果①正常晚期妊娠蜕膜组织中CD56+CD3-NK细胞占蜕膜单个核细胞(DMC)7.82%±3.3%,ICP患者蜕膜中CD56+CD3-NK细胞含量为38.15%±5.1%(P<0.05);②正常晚期妊娠蜕膜组织中CD56+CD16-、CD56+CD16+NK亚群含量明显低于ICP组,差异有显著性(P<0.05);③ICP组蜕膜组织中NK细胞杀伤活性高于正常晚期妊娠蜕膜组,差异有显著性(P<0.05)。结论NK细胞是妊娠晚期蜕膜组织中的主要淋巴细胞,其亚群失衡及功能异常可能是ICP发病的重要原因。Objective To determine the number, activity and phenotype of decidual natural killer （NK） cells in patients with Intrahepatie eholestasis of pregnancy （ICP）. Methods 15 patients with ICP were studied ,and 15 patients with normal third pregnant women were selected as control group. The percentage of NK cells and their subsets in deeiduas were detected using flow eytometrie analysis. The NK cells activity was measured by improved lactic acid dehydragenase releasing assay. Results Compared with control group, the percentage of CD56^＋CD3 ^- NK cells in deeidual mononuelear cells （DMC） of ICP patients was significantly higher than that in normal third pregnant women. The percentage of CD56^＋ CD16^-NK subset in ICP and normal third pregnant women was 38. 15±5.1% and 27.82 ± 3.3% respectively, which had a significant difference （P 〈 0. 05 ）. The percentage of CD56^＋ CD16 ^＋ NK subset in ICP and NP was 8. 46% ±1.21% and 4. 77% ± 1.32% respectively. The activity of decidual NK cells in ICP patients were higher than that of normal third pregnant women （ P 〈 0. 05 ）. Conclusions NK cell is predominant lymphocyte in normal decidua and plays an important role in maintaining successful pregnancy. Abnormally raised activity and disbalaneed CD56 ^＋ CD16 ^＋ ,CD56^＋ CD16^- subsets of decidua NK cell are associated with ICP and may be the one of reasons for leading to ICP.

关 键 词：蜕膜 杀伤细胞 天然 流式细胞术 胆汁淤积 肝内 妊娠并发症 

分 类 号：R714.2[医药卫生—妇产科学] R575[医药卫生—临床医学]