小鼠血吸虫病肝组织中ESM-1的表达及黄芪总苷对其影响  

The expression of endothelial cell specific molecule-1 in mice liver tissues with schitosomiasis japonica and effect of astrogalosides on it

作　　者：张岩平[1] 吴强[1] 丁向东[1] 左莉[2] 张晴[1] 王红群[1] 汪渊[2] 

机构地区：[1]安徽医科大学病理学教研室 [2]安徽医科大学分子生物学中心实验室和生物化学教研室,安徽省基因研究重点实验室,合肥230032

出　　处：《安徽医科大学学报》2008年第3期283-287,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高校自然科学基金重点资助项目(编号:2006KJ092A)

摘　　要：目的观察小鼠血吸虫病肝组织中内皮细胞特异性分子-1(ESM-1)的变化及黄芪总苷(AST)对其影响。方法ICR小鼠120只随机分为AST高剂量(20mg/kg.d-1)组,AST低剂量(10mg/kg.d-1)组,阳性药(护肝片,540mg/kg.d-1)组、模型对照组,每只实验小鼠感染血吸虫尾蚴30条,均于感染40d后以吡喹酮(500mg/kg.d-1)治疗2d。同时设30只小鼠为正常对照组。分别于感染后6、10和14周末,每组随机处死10只小鼠,取肝脏组织作常规病理学检测并制作组织芯片,采用免疫组织化学和原位分子杂交技术检测ESM-1mRNA及蛋白。结果①抗ESM-1蛋白抗体在血吸虫肝病组织中的炎细胞、成纤维细胞、胆管上皮细胞、血管内皮细胞、肝细胞均呈不同程度的阳性反应,ESM-1mR-NA主要在炎细胞、胆管上皮细胞、血管内皮细胞和肝细胞表达,成纤维细胞未检测出ESM-1mRNA;②10周和14周末AST两个剂量组ESM-1的蛋白水平低于模型组(P<0.01),10周末AST高、低剂量组ESM-1mRNA阳性细胞计数低于模型组(P<0.01)。结论小鼠血吸虫病肝组织中,炎细胞可产生ESM-1,成纤维细胞可能存在ESM-1受体。ESM-1可能通过旁分泌作用参与血吸虫肝纤维化过程,黄芪总苷对ESM-1的抑制作用可能是其抗纤维化的机制之一。Objective To observe ESM-1 alteration in mice liver tissues with schitosomiasis japonica and effect of astrogalosides on it. Methods One hundred and twenty ICR mice were randomly divided into four groups： high dose astrogalosides group （20 mg/kg per day）, low dose astrogalosides group （ 10 mg/kg per day）, positive drug control group （liver protection tablet, 540 mg/kg per day） and model group. Each experiment mouse was infected with thirty schistosome cercarie. Forty days after infection, all mice for the experiment were treated with praziquantel （500 mg/kg per day） for two days. Thirty healthy ICR mice were used in this experiment as normal control group. Ten mice in each group were sacrificed randomly at the end of the 6th, 10th and 14th weeks, respectively. Hepatic tissues were sampled for pathologic study. ESM-1 protein and its mRNA were detected with immunohistochemistry and in situ hybridization on tissue microarray sections, respectively. Results ① Positive staining for the anti-ESM-1 antibodies was found in inflammatory cells, fibroblasts, bile duct epithelial cells, vascular endothelial cells and hepatocytes in liver tissues with schistosomiasis; Positive staining for the ESM-1 mRNA was mainly located in inflammatory cells, bile duct epithelial cells, vascular endothelial cells and hepatocytes, however, no posi- tive staining for the mRNA was shown in fibroblasts. ② At the end of the 10th and 14th weeks, the levels of ESM- 1 protein in both AST groups were significantly lower than those of model group （ P 〈 0. 01 ）, at the end of the 10th, ESM-1 mRNA positive cell counts of high dose, low dose AST groups were significantly lower than those of model group（P 〈 0. 01 ）. Conclusion Inflammatory cells might produce ESM-1 in mice hepatic tissues with schistosomiasis japonica, while there might exist receptors of ESM-1 in fibroblasts. ESM-1 might participate in schistosomal liver fibrosis via its paracrine action through inflammatory cells to fibroblasts. The depression effect

关 键 词：血吸虫 日本 肝硬化 黄芪苷/药理学 内皮细胞 基因表达 

分 类 号：R383.4[医药卫生—医学寄生虫学] R575.2[医药卫生—基础医学]