重瓣矮牵牛的组织培养  

Double petunia common petunia's tissue culture

作　　者：李瑛[1] 

机构地区：[1]哈尔滨学院,黑龙江哈尔滨150040

出　　处：《国土与自然资源研究》2008年第2期84-86,共3页Territory & Natural Resources Study

摘　　要：重瓣矮牵牛色泽艳丽,观赏性强,是重要的彩化花卉品种,但由于其不能正常结实,繁殖成活率低,扦插也不易成活,所以采用组织培养的方法进行繁殖;利用基本培养基MS、细胞分裂素(6-BA)、生长素IBA,以植株叶片为材料,在一定条件下采用不同的浓度配比,观察植物叶片经过消毒—发芽—生根—开花的生长过程,选出最佳的植物繁殖配比浓度;在短期达到快速繁殖的目的。Double petunia with its bright color has very strong attraction as an unusual ornamental plant For this reason, it becomes an indispensable kind of flower and greenery which are used in colorful plant decoration. But it can＇ t fructify normally and has a low survival rate of propagation. Meanwhile, it is difficult for it to live by the way of cutting propagation. As a result double petunia is usually propagated by the method of tissue culture. In the experiments, with the plant leaves as materials and under certain conditions, basic culture medium MS, cytokinin 6-BA and growth factor IBA are used at various concentration ratio to make double petunia propagate. Through the observation of the plant leaves＇ growth process of disinfection, sprouting, rooting and blooming, the best concentration ratio of plant propagation is selected. This method can he used to achieve the goal of rapid propagation in short period and to create economic benefit for the development of the flower and greenery r market.

关 键 词：重瓣矮牵牛 基本培养基MS 细胞分裂素(6-BA) 生长素IBA 成活率 

分 类 号：S359[农业科学—作物栽培与耕作技术]