日粮锌水平对生长肉兔生产性能、血清肝脏抗氧化酶活性和金属硫蛋白-1基因表达的影响  

Effects of Dietary Zn Levels on the Production Performance,Antioxidase Activity in Serum and Liver,and Metallothionein(MT)-1 mRNA Expression of Growing Rabbits

作　　者：徐振华[1] 李福昌[1] 秦应和[2] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]中国农业大学动物科技学院,北京100193

出　　处：《动物营养学报》2008年第3期337-342,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：“十一五”国家科技支撑计划(2006BAD14B07-5-6);2007年公益性行业(农业)科研专项经费资助(nyhyzx07-040)

摘　　要：本文旨在探讨日粮不同锌添加水平和锌源对2～3月龄生长肉兔生产性能、血清肝脏抗氧化酶活性和金属硫蛋白-1(MT-1)基因表达的影响。选用2月龄新西兰肉兔70只,随机分为7个处理,对照组饲喂基础日粮,试验组在基础日粮中分别以硫酸锌和蛋氨酸锌形式添加40、80和120mg/kg锌,预试期7d,正试期23d。结果表明:日粮锌添加水平对生长肉兔平均日增重(ADG)和料重比(F/G)无显著性影响(P>0.05),对平均日采食量(ADI)影响显著(P<0.05);不同锌水平对血清铜锌超氧化物歧化酶(CuZn-SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性影响显著(P<0.05),对肝脏CuZn-SOD和GSH-Px活性影响极显著(P<0.01);随日粮锌水平的增加,肝脏、肾脏MT-1mRNA相对表达丰度呈先升高后降低趋势(P<0.01),80mg/kg添加水平时2种锌源MT-1mRNA相对表达丰度均达到最高。MT-1mRNA相对表达丰度可以作为评价动物机体锌营养状况的敏感指标。To study the effects of dietary ZnSO4 and Zn-Met levels on production performance, CuZn-SOD in serum and liver, GSH-Px activities, MDA concentration and MT-1 mRNA expression of 2 to 3-month-old New Zealand growing rabbits. In the experiment, 1 basal diet and 6 trial diets （the basal diet with the supplementation of 40 , 80 and 120 mg/kg of ZnSO4 and Zn-Met, respectively） were prepared for the control and trial groups. Seventy two-month-old New Zealand rabbits were randomly allotted to seven treatments with ten rabbits for each. The trial lasted for 30 days, including 7 days for adaptation. The results showed that different Zn levels had no significant effects on the average daily gain （ADG） and the feed/gain ratio （F/G） （P〉0.05）, but significant effects on the average daily intake （ADI）（P〈0.05） ; The dietary Zn level could significantly improve the CuZn-SOD and GSH-Px activities in serum （P%0.05）, with extremely significant effect on the CuZn-SOD and GSH-Px activities in liver （P〈0.01） ; With the increase of Zn level, the relative MT-lmRNA expression abundance in liver and kidney increased at the beginning and then decreased （P〈0.01）,reaching the highest when 80 mg/kg of either Zn source was supplemented; In conclusion, the MT-1 mRNA expression abundance in liver could be adopted to evaluate the zinc nutritional status in growing meat rabbits; The appropriate dietary Zn level for 2 to 3-month-old growing meat rabbits was 80 mg/kg, while Zn-Met was more effective than ZnSO4.

关 键 词：锌 生长肉兔 生产性能 抗氧化酶 金属硫蛋白-1 MRNA 

分 类 号：S565.4[农业科学—作物学] S831.5