染料介导光氧化处理去细胞猪主动脉瓣的生物学特性  

Biological and Biomechanical Properties of Acellular Porcine Aortic Valve Stabilized by Dye Mediated Photo Oxidation

作　　者：刘洋[1] 孙国成[1] 刘珊珊[2] 谭红梅[1] 欧阳辉[1] 易定华[1] 刘维永[1] 

机构地区：[1]第四军医大学西京医院心脏外科,西安710032 [2]第四军医大学唐都医院超声诊断科,西安710038

出　　处：《中国胸心血管外科临床杂志》2008年第3期200-203,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA02A138);陕西省科技攻关资助项目[2007K11-03(1)]~~

摘　　要：目的探讨染料介导光氧化处理猪主动脉瓣膜的生物学特性,以期获得性能良好的新型生物瓣膜材料。方法经去细胞处理的猪主动脉瓣30个,随机分为3组。光氧化处理组(n=10):去细胞瓣膜经染料介导光氧化处理;戊二醛处理组(n=10):去细胞瓣膜采用戊二醛处理;去细胞对照组(n=10):去细胞瓣不做固定处理,置于生理盐水中备用。分别测定并比较3组瓣膜的形态学改变、组织学特点、瓣膜厚度、含水量、热皱缩温度、断裂强度和可溶性蛋白含量。结果光氧化处理组主动脉瓣呈淡蓝色,瓣膜质地柔软,弹性大,瓣叶无皱缩。光氧化处理组主动脉瓣瓣膜厚度较戊二醛处理组薄(0.26±0.09mmvs.0.38±0.08mm;P<0.05),组织含水量较戊二醛处理组大(86.30%±4.03%vs.71.10%±3.23%;P<0.05),热皱缩温度低于戊二醛处理组(76.30±0.70℃vs.87.70±0.30℃;P<0.05);瓣膜厚度、组织含水量和热皱缩温度与去细胞对照组比较差异无统计学意义。光氧化处理组断裂强度较去细胞对照组明显增大(17.33±2.65mPavs.9.11±0.95mPa,P<0.05),可溶性蛋白含量较去细胞对照组明显减少(0.039%±0.013%vs.0.107%±0.024%;P<0.05),而与戊二醛处理组比较差异无统计学意义。结论染料介导光氧化处理去细胞猪主动脉瓣有较好的生物学特性。Objective To investigate the biological and biomechanical characteristics of acellular porcine aortic valve with dye mediated photo oxidation so that a new and better bioprosthetic valve materials can be obtained. Methods Thirty porcine aortic valves were divided into three groups with random number table. Acellular valves （n= 10） were stabilized by dye mediated photo oxidation in dye mediated photo oxidation group ; acellular valves （n= 10） were stabilized by glutaraldehyde in glutaraldehyde group; and acellular valves （n= 10） were acellularized only in acellular valves group. Thickness, appearance, histology, water content, shrinkage temperature, breaking strength and soluble protein level of acellular porcine aortic in three groups were tested respectively. Results There were light blue, soft, flexible and unshrinking valves in dye mediated photo oxidation group. Compared to valves in glutaraldehyde group, valves in dye mediated photo oxidation group had lighter thickness（0.26±0.09mm vs. 0. 38±0.08mm, P〈0.05）, more water content（86.30%±4.03% vs. 71.10%±3. 23%, P〈0.05）, and lower shrinkage temperature （76.30±0.70℃ vs. 87.70±0. 30℃, P〈0. 05）; while these indexes had no statistically significant differences compared to those in acellular valves group. At the same time, compared to valves in acellular valves group, valves in dye mediated photo oxidation group had more breaking strength（17.33±2.65 mPa vs. 9.11±0.95 mPa, P〈0.05） and lower soluble protein level（0.039%±0.013% vs. 0.107%±0.024%, P〈0.05）; while these indexes had no statistically significant differences compared to those in glutaraldehyde group. Conclusion Acellular porcine aortic valve stabilized by dye mediated photo oxidation has nice biological and biomechanical characteristics.

关 键 词：染料介导光氧化 去细胞 生物瓣膜 生物学特性 

分 类 号：R318.08[医药卫生—生物医学工程]