大肠埃希菌新的氨基糖苷类修饰酶基因型别研究  

New Genotypes for Aminoglycoside-modifying Enzymes in Escherichia coli

作　　者：俞莲花[1] 潘春琴[1] 胡大康[1] 刘池波[1] 张瑾[1] 

机构地区：[1]浙江省台州学院医学院附属台州市立医院检验科,浙江台州318000

出　　处：《中华医院感染学杂志》2008年第6期757-759,共3页Chinese Journal of Nosocomiology

基　　金：椒江区科委(62086)

摘　　要：目的研究大肠埃希菌氨基糖苷类修饰酶基因aac(6′)-Ⅰb各亚型的流行情况。方法对氨基糖苷类修饰酶基因aac(6′)-Ⅰb阳性的菌株进行DNA序列测定,测得序列与已在美国国立生物信息中心(NCBI)登录的aac(6′)-Ⅰb基因家族序列比对,确定型别。结果6株aac(6′)-Ⅰb阳性的大肠埃希菌,经DNA序列测定,其中4株为经典型,1株为aac(6′)-Ⅰb-Cr型,另外1株为aac(6′)-Ⅰb新亚型。结论大肠埃希菌氨基糖苷类修饰酶基因aac(6′)-Ⅰb至少存在3种型别,以经典型为主,同时存在aac(6′)-Ⅰb-Cr型和aac(6′)-Ⅰb新亚型。OBJECTIVE To study the prevalence of aac （6＇）-Ⅰ b with its subtypes, one kind of gene for aminoglycoside-modifying enzymes in Escherichia coll. METHODS DNA sequences were analyzed in those strains with aac（6＇）-Ⅰ b, compared to its family marked in NCBI. Thus, its subtypes were determined. RESULTS Four strains were classical on genotype, the other one with aac（6＇）-Ⅰ h-Cr, and the last one with a new subtype among the 6 strains of E. coli with aac（6＇）-Ⅰ b. CONCLUSIONS There exist at least 3 subtypes of aac（6＇）-Ⅰ b, one kind of gene for aminoglycoside-modifying enzymes in local strains of E, coll. Among those subtypes, the classical type is the main, accompanied by aac（6＇）-Ⅰ b-Cr and its new subtype.

关 键 词：大肠埃希菌 氨基糖苷类修饰酶 基因型别 

分 类 号：R378.21[医药卫生—病原生物学]