深圳海域斑节对虾野生种群线粒体控制区序列的多态性分析  

Analysis on genetic diversity of mtDNA control region sequences of the wild population of Peneanus monodon from Shenzhen′s sea area

作　　者：姜永杰[1] 周发林[1] 黄建华[1] 马之明[1] 江世贵[1] 

机构地区：[1]中国水产科学研究院南海水产研究所

出　　处：《南方水产》2006年第1期54-57,共4页South China Fisheries Science

基　　金：国家"863"高技术研究发展计划项目(2003AA603120)

摘　　要：采用聚合酶链式反应技术对深圳斑节对虾种群的20个个体进行了分析，通过对mtDNA的控制区基因序列进行扩增，获得了大小约为650bp的扩增产物。PCR产物经纯化后进行序列测定，得到了526bp的核苷酸序列。用Clustal_X排序软件对控制区序列进行了对位排列。通过Mega软件对所得线粒体控制区序列的片段进行比较，共检测出114个碱基存在变异，其中包括84个简约信息位点，5个碱基存在插入／缺失；并用Mega的“Pairwise distance”计算个体间的相对遗传距离。结果表明：其序列差异（转换＋颠换）在0．010-0．154之间，得出20个个体有20种单倍型；并构建了UPGMA和NJ系统树。运用DNASP软件计算所得该群体核苷酸多样性（R）和平均核苷酸差异数（K）分别为0．05278和27．500。研究结果表明：深圳斑节对虾野生种群控制区序列个体变异程度很大，该种群的遗传多样性水平很高，适合于群体内及群体间不同个体的遗传多样性分析。The PCR technique was used to analyze the mtDNA control region sequences in 20 individuals of P. monodon in Shenzhen＇ s sea area. A series of nucleotide fragments with about 650 bp were obtained. The PCR products were purified and sequenced. As a resuit, a series of 526 bp nucleotide sequences of partial control regions were obtained. The 20 sequences were arranged in counterpoint by Clustal_X software. And then, comparing the control region fragments with each other by Mega software, 114 sites were variable a-mong all the partial mitochondrial control region sequences including 84 parsimonious message sites and five deletion / insertion sites. The relative genetic distances were calculated among individuals by ＂Pairwise distance＂ method of Mega. The results indicated that the differentiation of the 20 sequences ranged from 0. 010 to 0. 054 and showed 20 kinds of haplotypes among 20 individuals. The UPGMA and NJ phylogenetic trees were established by the cluster analysis. The number of polymorphic sites （S） was 114; the nueleotide diversity （Pi） and the average number of nucleotide differences （K） were 0. 05278 and 27. 500, respectively. It can be concluded that the variation in mtDNA control region sequences of P. monodon in Shenzhen＇s sea area was greater relatively. So the genetic diversity of P. monodon in Shenzhen＇s sea area was higher relatively. As a result, mtDNA control region sequences can be used to scan genetic diversity of populations.

关 键 词：斑节对虾 线粒体 控制区序列 遗传多样性 

分 类 号：Q523.8[生物学—生物化学] S917[农业科学—水产科学]