中华倒刺鲃血清凝集素的研究  

Study on an agglutinin isolated from serum of Spinibarbus sinensis

作　　者：张黎[1] 刘莉[1] 袁媛[1] 顾继锐 吴江[2] 谢显连 徐恒[1] 

机构地区：[1]四川大学生物资源与生态环境教育部重点实验室,四川成都610064 [2]通威股份通威水产科技有限公司,四川成都610081

出　　处：《南方水产》2006年第6期39-44,共6页South China Fisheries Science

基　　金：四川省科技攻关项目(05NG002-002-4)通威股份科技发展项目

摘　　要：从中华倒刺鱼巴(Spinibarbus sinensis)血清分离到一种天然的凝集素,能够凝集多种不同的抗原物质,其组成成分为糖蛋白,具有耐热和耐酸碱性,对β-巯基乙醇敏感。凝集反应受温度、PBS离子浓度以及时间等因素的影响。经糖的专一性抑制实验显示,蔗糖和乳糖对凝集反应有抑制作用,说明这2种糖分子是凝集素在识别和凝集过程中起一定作用的糖分子。A natural occurring agglutinin, which was detected from serum of Spinibarbus sinensis, was found to have the ability of ag- glutinating different kinds of antigen. It has the characteristic of glycoprotein. The agglutinin was stable when exposured to the heat （ 60℃ ） for l h and acid （ pH = 2） or alkaline （ pH = 10） for 24 h, respectively, but was sensitive to the treatment of 1% β-mercaptoethanol. Its agglutinating activity was influenced by the temperature, reaction time and concentrating of ion of PBS. The agglutinating activity could be inhibited by the splitting of lactose and sucrose. The results demonstrated the sugar referred above played an important role in agglutinating reaction.

关 键 词：中华倒刺鲃 凝集素 理化性质 

分 类 号：Q592.1[生物学—生物化学]