过敏性紫癜患儿血淋巴细胞亚群变化的意义  

Significance of Changes of Lymphocyte Subtypes in Children with Henoch-Schonlein Purpura

作　　者：孙建新[1] 毕玉娜[1] 朱静[1] 季正华[1] 封其华[1] 倪征[1] 程江[1] 徐琴英[1] 王丽峰[1] 闵月[1] 李晓忠[1] 

机构地区：[1]苏州大学附属儿童医院肾内科,江苏苏州215003

出　　处：《实用儿科临床杂志》2008年第9期665-667,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(30271234)

摘　　要：目的探讨过敏性紫癜(HSP)患儿血淋巴细胞亚群变化的意义。方法采用双标法在流式细胞仪上检测61例住院HSP患儿血淋巴细胞亚群CD3+、CD3+CD4+、CD3+CD8+、CD4+/CD8+、CD3-CD19+、CD3-(CD16+56+)、CD19+CD23+水平,并与同期19例同龄健康儿童作对照;采用速率法应用HIT蛋白自动分析仪测定其中53例HSP患儿血清IgA及其淋巴细胞亚群;采用速率法、应用OLYMPUSAU400自动分析仪测定其中45例HSP患儿尿微量清蛋白、尿肌酐、N-乙酰-β-氨基-葡萄糖苷酶(NAG)。应用SAS6.12软件进行统计学分析。结果HSP组与健康对照组比较血CD3+CD4+、CD4+/CD8+、CD3-(CD16+56+)明显降低(Pa<0.05),CD3-CD19+、CD19+CD23+明显升高,CD3+、CD3+CD8+无显著性差异。血清IgA水平与淋巴细胞亚群无相关性(r=0.224P>0.05),以尿微量清蛋白/尿肌酐(UmAlb/Cr)(mg/g)=30为界限,NAG以11.9U/L为界限,分别将淋巴细胞亚群分二组,结果显示UmAlb/Cr>30与≤30组比较,NAG>11.9与≤11.9组比较,CD19+CD23+均明显升高[(6.71±0.80)vs(5.78±0.46),(9.36±1.14)vs(5.44±0.50)Pa<0.05]。结论HSP急性期存在免疫功能紊乱,尤其是活化的B淋巴细胞(CD19+CD23+)明显升高,并且其可能与肾损伤程度有一定关系。Objective To study the significance of changes of lymphocyte subtypes in children with Henoch - Schonlein purpura （HSP）. Methods Flow cytometric （ FCM ） analysis was performed to detect the changes of lymphocyte subtypes levels including CD3^＋ , CD3^＋ CD4^＋ ,CD3^＋ CD8^＋ ,CD4^ ＋/CD8^ ＋ , CD3ˉ CD19^ ＋ , CD3ˉ （ CD16＋56 ^＋ ） , CD19^＋CD23^＋ in 61 children with HSP. HIT protein autoanalyser was used to measure serum immunoglobin in 53 of these children and analyze relatationship between them. OLYMPUSAU 400 autoanalyser was used to quantity the urine creatinine,urinary albumin and N - acetyl - β - D - glucosaminidase（NAG） in 45 HSP children. SAS 6.12 software was used to analyze the data. Results CD3^ ＋ CD4^ ＋ , CD4^＋/CD8^＋ , CD3ˉ （CD16 ＋56^＋ ） cell lymocytes in HSP were lower than those in controls （ Pa 〈 0.05 ） , CD3ˉ CD19^＋ , CD19^＋ CD23^＋ cell were found higher in HSP , compared with the controls （ Pa 〈 0.05 ）. There was no correlation between serum immunoglobin and lymphocyte subtypes （ r = 0. 224 P 〉 0.05 ）. Tracing albumin in urine/urine creatinine （ UmAlb/ Cr） （mg/g） = 30 and the limit of NAG to 11.9 U/L for boundaries, respectively, lymphocyte subsets will be divided into 2 groups,CD19^＋ CD23^＋ lymphocyte inereased in UmAlb/Cr 〉 30 and NAG 〉 11.9 than UmAlb/Cr ≤ 30 and NAG ≤ 11.9 [ （ 6.71 ± 0.80 ） vs （ 5.78 ± 0.46 ）, （9.36 ± 1.14 ） vs （5.44 ± 0.50 ） Pa 〈 0.05 ]. Conclusions Lymphocyte disturburance is involved in the pathophysilogic process of HSP and especially CD19^＋ CD23^＋ cell is found higher in HSP and may participate in the renal injury.

关 键 词：紫癜 过敏性 淋巴细胞亚群 CD19^+CD23^+ 

分 类 号：R725.5[医药卫生—儿科]