细胞壁降解酶在落叶松-杨栅锈菌与寄主互作过程中的作用  

Role of Cell Wall-Degrading Enzymes in Interaction of Poplar and Melampsora larici-populina

作　　者：田呈明[1] 赵鹏[2] 曹支敏[2] 

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083 [2]西北农林科技大学林学院,杨凌712100

出　　处：《林业科学》2008年第5期79-83,共5页Scientia Silvae Sinicae

基　　金：国家自然基金项目(30471394);教育部留学回国人员科研启动基金资助项目

摘　　要：对落叶松-杨栅锈菌入侵不同抗性寄主过程中的细胞壁降解酶活性进行测定,明确聚甲基半乳糖醛酸酶(PMG)、果胶甲基反式消除酶(PMTE)、羧甲基纤维素酶(CX)、β-葡萄糖苷酶(β-G)4种降解酶在该菌入侵中的作用。研究发现:抗病和感病组合中PMG均在接种后第2天和第5天出现2个活性高峰,在第5天时达到最大值。PMTE、CX和β-G活性分别第3、2和3天达到活性高峰,其中PMG和PMTE的活性高,而CX和β-G的活性则相对较低。在感病组合内PMG、PMTE、CX、β-G活性较抗病组合大,表明这4种细胞壁降解酶对该菌入侵起一定的作用。The activities of cell wall-degrading enzymes in poplar cuhivars infected by Melampsora larici-populina were studied. The results showed that PMG, PMTE, Cx and β-glucosidase played roles during infection. After inoculation, the activity of PMG in both susceptible and resistant cuhivars had two peak values respectively in 2 dpi and 5 dpi. The activities of PMTE and β-glucosidase had their peak values in 3dpi, and Cx in 2 dpi. The activities of PMG and PMTE were higher while the activities of Cx and β-glucosidase were relatively lower. Activities of these cell wall-degrading enzymes were significant higher in susceptible cuhivars than those in resistant cuhivars. The results suggested that these cell wall-degrading enzymes played certain roles in infection of M. larici-populina.

关 键 词：落叶松-杨栅锈菌 细胞壁降解酶 杨树 

分 类 号：S763.1[农业科学—森林保护学]