银星秋海棠组织培养的研究  

Study on the Tissue Culters of Begonia argenteo-guttata

作　　者：李任珠[1] 卢海强 邢增盛 

机构地区：[1]海南大学农学院,海口570228

出　　处：《海南大学学报（自然科学版）》1997年第4期331-336,共6页Natural Science Journal of Hainan University

摘　　要：采用不同培养基对银星秋海棠进行组织培养,结果表明：诱导银星秋海棠外植体产生愈伤组织的最佳培养基是：MS＋BA0.5mg/L＋NAA0.2mg/L;诱导意伤组织分化出芽的最佳培养基是：MS＋BA1mg/L＋NAAO.1mg/L;诱导生根的最佳培养基是：1/2MS＋NAAO.2mg/L.不同外植体产生愈伤组织的能力大小顺序为叶片＞叶柄＞嫩茎.试管苗移栽对基士的选择不严,成活率均较高.Begonia argenteo- guttata was cultured in different media. The experimental results demonstrated that the MS medium supplemented with 0.5mg/L of BA and 0.2 mg/L of NAA had ideal effects on the inducing of callus from begonia argenteo- guttata explanta. The optimum medium of inducing callus to adventitious buds was MS + BA 0. 1mg/L+ NAA 0. 1mg/L. In addition, found that 1/2 MS + NAA 0.2 mg/L had the best induced effect on root formnation, ability order of callus induction was leaf＞ peduncle ＞ young stem; the transplantation of tube plantlets didn' t need spocial matrix; in all matrixes tested the survival the of planilets was fairly high.

关 键 词：花卉 银星秋海棠 组织培养 培养基 

分 类 号：S680.3[农业科学—观赏园艺] Q949.759.7[农业科学—园艺学]