HBsAg阳性患者乙型肝炎病毒表面大蛋白的检测及其意义  

Clinical Significance of Measuring Large Susrface Protein of Hepatitis B Virus in Patients with HBsAg-positive Blood

作　　者：吴正林[1] 陆学东[1] 钟小强[1] 凌利芬[1] 林广城 肖桂初 

机构地区：[1]深圳市第四人民医院检验医学部,广东深圳518033

出　　处：《现代检验医学杂志》2008年第3期49-51,共3页Journal of Modern Laboratory Medicine

基　　金：深圳市福田区科技计划项目(FT200641)

摘　　要：目的探讨检测乙型肝炎病毒表面大蛋白(LHBs)用于反映HBsAg阳性患者体内乙型肝炎病毒复制的临床意义。方法采用酶联免疫吸附实验(ELISA)和荧光定量PCR法对1066例HBsAg阳性血清标本进行LHBs及HBVDNA检测。结果1在1066例HBsAg阳性血清中HBVDNA阳性率为63.23%(674/1066),LHBs的阳性率为66.42%(708/1066),二者比较无显著性差异(χ2=2.240,P>0.05),两者总体符合率为91.93%;2不同HBV-M模式的HBV-DNA与LHBs检出结果均无显著性差异(P>0.05)。3LHBs含量与HBVDNA拷贝数呈正相关(r=0.927,P<0.001)。结论血清中LHBs含量与HBV-DNA的拷贝数具有较好的相关性,LHBs能够反映HBV的复制情况。Objective To explore the significance of measuring large surface protein（LHBs） of hepatitis B virus in the diagnosis of viral repication. Methods Enzyme linked immunosorbent assay（ELISA） methods were used to examine the LHBs and quantitative real-tlme PCR methods were used to detect the HI3V DNA of 1 066 HBsAg-positive serum samples. Resuits ①No significant difference of positive rate was observed between HBV DNA 63. 23%（674/1 066）and LHBs 66. 42% （708/1 066）（X^2=2. 240,P〉0.05） in 1 066 HBsAg-positive serum samples,the collectivity accord rate was 91.93% between LHBs and HBV-DNA. ②The expression of HBV-DNA and LHBs were not significantly different among various patterns of HBV serological marks either （P〉0. 05）. ③Serum LHBs levels were correlated with the serum HBV DNA copies （r=0. 927,P〈0. 001）. Conclusion There is a perfect corelation between the copies of HBV-DNA and the levels of LHBs,and LHBs expression can reflect the replication of HBV.

关 键 词：HBV 大蛋白 HBSAG HBV—DNA 

分 类 号：R512.62[医药卫生—内科学] R446.62[医药卫生—临床医学]