葛根素对大鼠肝脏缺血再灌注损伤的抗氧化作用及对部分生化指标的影响  

Anti-oxidation Role of Puerarin Against Hepatic Ischemia-reperfusion Injury and Its Effect on some Biochemical Indicators in Rats

作　　者：谭业克[1] 晋红梅[2] 

机构地区：[1]泰安市中心医院检验科,山东泰安271000 [2]泰山医学院附属医院输血科,山东泰安271000

出　　处：《现代检验医学杂志》2008年第3期63-65,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨葛根素对大鼠缺血再灌注损伤肝脏的抗氧化作用及对部分生化指标的影响。方法建立大鼠全肝缺血再灌注模型,60只大鼠随机分为三组,每组20只,分别为假手术对照组(A组)、肝缺血/再灌注组(B组)和肝缺血/再灌注加葛根素治疗组(C组),分别在肝缺血前、缺血45min、再灌注45min共3个时相点,检测血浆超氧化物歧化酶(SOD)活性、黄嘌呤氧化酶(XO)活性、丙二醛(MDA)浓度以及血清谷丙转氨酶(ALT)活性。结果肝缺血/再灌注组,血浆XO,MDA及ALT显著高于假手术对照组、SOD明显低于假手术对照组(P<0.05或P<0.01);而葛根素治疗组与缺血/再灌注组比较,上述指标均有显著性差异(P<0.05或P<0.01)。结论葛根素可通过降低氧自由基水平(增强SOD活性、减弱XO活性)拮抗脂质过氧化反应(降低MDA浓度),从而减轻肝脏缺血再灌注损伤。Objective To investigate the anti-oxidatlon role of puerarln against hepatic ischemia-reperfusioon injury and its effect on some biochemical indicators in rats. Methods 60 cats were selected and divided into three groups randomly（n= 20） ,sham operated group （A）,ischemia-reperfusion group （B） and ischemia reperfusion/puerarin group （C）. Changes of several parameters which included malondialdehyde （MDA）,superoxide dismutase （SOD）,xanthine oxidase（XO） and alanine aminotransferase（ALT） were measured before ischemia;45 minutes after ischemia and 45 minutes after reperfusion in plasma. Results XO and MDA increased as well as SOD decreassed at 45 minutes after reperfusion （P〈0.05 or P〈 0.01） in plasma. Differences of each items between group B and C were significant （P〈0. 05 or P〈0.01）. Conclusion Puerarin may attenuate hepatic ischemia-reperfusion injury by dropping oxygen free radicals level （reising SOD activity and dropping XO activity） and antagonizing lipid peroxidation（reducing MDA content）.

关 键 词：缺血/再灌注损伤 肝脏 氧自由基 葛根素 

分 类 号：R285.5[医药卫生—中药学] R446.112[医药卫生—中医学]