慢性束缚应激对大鼠脑内Fas、FasL含量的影响  

Effects of Chronic Immobilization Stress on the Expression of Fas/FasL in the Brain of Rats

作　　者：朱婉儿[1] 张蓉[1] 胡长春[1] 董逢泉[1] 吴丽霞[1] 陈芝芸[2] 

机构地区：[1]浙江大学医学院医学心理学,杭州310058 [2]浙江省中医院消化病研究室,杭州310006

出　　处：《心理学报》2008年第6期723-728,共6页Acta Psychologica Sinica

基　　金：浙江省自然科学基金(2005Y013);教育部留学回国人员科研启动基金(J20040252)资助项目

摘　　要：为探讨慢性束缚应激对大鼠脑内不同部位Fas/FasL系统表达的不同影响。将24只雄性SD大鼠随机分为束缚应激组、装置对照组和正常对照组(n=8)。分别对三组大鼠给予相应的干预14天,用Western-blotting方法测定应激后大鼠大脑前脑皮质、内嗅皮质以及海马区域Fas、FasL蛋白含量。结果表明,应激后各脑区三组大鼠Fas含量差异具有统计学意义(p<0.01),束缚应激组明显高于正常对照组或装置对照组(p<0.01)。在海马区域,三组大鼠FasL含量差异具有统计学意义(p<0.01),束缚应激组大鼠FasL蛋白水平显著高于正常对照组或装置对照组(p<0.01)。三组大鼠自身大脑前脑皮质、内嗅皮质以及海马Fas含量的差异比较均无统计学意义(p>0.05)。束缚应激组大鼠海马的FasL含量高于前脑皮质和内嗅皮质(p<0.05);正常对照组或装置对照组自身不同脑区间FasL含量的变化无统计学意义。提示慢性束缚应激能诱导大鼠脑内Fas/FasL系统表达水平的改变,对不同的脑区,其影响程度明显不同。there exists a substantial body of evidence suggesting that chronic stress can increase susceptibility to diseases such as depression and posttraumatie stress disorder. Chronic stress induces neuronal atrophy and death in the cortex as well as in the hippoeampus. Proto - oncogene protein kinase B （PKB）, also known as Akt, plays a central role in a signaling pathway whose various components have been linked to cellular survival. In recent years, Akt as well as one of its downstream targets Forkhead transcription factors （forkhead homologne in rhabdomyosarcoma, （FKHR）） and the Fas/FasL system have emerged as cardinal pathways underlying cellular survival and opposing apoptosis in neurons. In our previous experiments, we observed that repeated immobilization stress exposure can change the levels of phosphorylation of Akt and FKHRL1 in the rat hippoeampus. However, the activation of the Fas/FasL system following chronic stress in the brain remains poorly defined. We hypothesized that repeated immobilization stress exposure may change the levels of the Fas/FasL system in the brains of rats. The purpose of the present study was to detect the effects of chronic immobilization stress on the expression of Fas/FasL in the prefrontal cortex, entorhinal cortex, and hippocampus in rats. Twenty four male Sprague - Dawley rats （280 ± 20g） were purchased from Shanghai Laboratory Animal Center, Chinese Academy Sciences, and they were used for all experiments. These rats were randomly divided into three groups - the immobilization stressed group, apparatus control group, and control group. Each group comprised eight rats. The rats were housed in groups of four, under standard laboratory conditions in temperature - controlled rooms （24℃）, and maintained under a 12 - h fight/dark cycle （lights on at 0800 hours） with food pellets and water available ad libitum. Rats from the immobilization stressed group were immobilized by fixing a board on their backs for an hour each day for 14 consecutive days. The appa

关 键 词：束缚应激 大脑前皮质 内嗅皮质 海马 FAS FASL 

分 类 号：B845[哲学宗教—心理学]