长臀AFLP分析体系的建立  

The establishment of AFLP analysis system in research of armorhead catfish,Cranoglanis bouderius

作　　者：程飞[1] 叶卫 叶富良[1] 刘波[3] 

机构地区：[1]广东海洋大学,广东湛江524025 [2]广东罗非鱼良种场,广东广州519320 [3]湖北师范学院,湖北黄石435002

出　　处：《南方水产》2007年第4期46-51,共6页South China Fisheries Science

摘　　要：以珠江长臀(Cranoglanis bouderius)为试验材料,建立了长臀扩增酶切片段长度多态性(AFLP)分析体系,对其分析过程中的DNA提取、双酶切、单酶切、连接、预扩增、选择性扩增、银染等步骤进行了检测,并对反应条件进行了优化。用PstI/TaqI、EcoRI/MseI等2组双酶切组合构建长臀的AFLP指纹图谱,条带清晰可辨,扩增信号强,得到清晰的长臀AFLP指纹图谱。AFLP （amplified fragment length polymorphism） analysis system was established in research of Pearl River armorhead catfish, Crartoglanis bouderius. The several steps for AFLP analysis were examined, including DNA extraction, double-restriction endonuclease reaction, single-restriction endonuclease reaction, adadpter ligation reaction, preamplification reaction, selective amplification reaction and Ag-NORs reaction. The AFLP fingerprinting experiments of C. bouderius with primer combination, E ＋ AAG/M ＋ CTG and P ＋ATT/T ＋ACA. were performed, which had about 50 clear and bright amplification bands.

关 键 词：长臀鮠 扩增酶切片段长度多态性(AFLP) 条件优化 DNA指纹 

分 类 号：Q755[生物学—分子生物学]