^(125)I标记重组MIF在炎症模型小鼠体内的生物学分布  

Biodistribution of ^(125)I Labeled Recombinant Macrophage Migration Inhibitory Factors in Inflammatory Model of Mice

作　　者：杨红[1] 梁婷[1] 宋静[1] 张超[1] 侯桂华[1] 

机构地区：[1]山东大学医学院实验核医学研究所,山东济南250012

出　　处：《同位素》2008年第2期101-104,共4页Journal of Isotopes

摘　　要：为初步评价125I-rMIF(重组巨噬细胞移动抑制因子,Recombinant Macrophage Migration Inhibition Factor,rMIF)在炎症显像中的应用价值,研究了125I-rMIF的稳定性、特异性及在炎症模型小鼠体内的生物学分布规律。结果显示,125I-rMIF的标记率为96.5%,室温下48 h内其生物学性质稳定。体内生物学分布研究显示,125I-rMIF主要由肝脏和肾脏代谢,血液清除较快。尾静脉注射125I-rMIF 0.5、1、6、24 h后,炎症肢体(靶)与对侧健肢(非靶)的放射性摄取比(T/NT)分别为1.42、1.35、2.18和2.05。以上结果表明,125I-rMIF具有在活体内炎症定位导向能力,但在早期优越性不明显,6 h后效果较佳,因此对隐匿性或亚急性炎症病灶的诊断具有潜在的临床价值。To evaluate ^125I labeled recombinant macrophage migration inhibitory factors （rMIF） for the scintigraphic imaging of inflammation, rMIF was labeled with ^125I by Iodogen method. ^125I-rMIF was isolated by Sephadex G25 column. The stability, immune specificity of ^125I-rMIF and its biodistribution in inflammatory model of mice were studied. The labeling yield of ^125I-rMIF was 96.5 %. It was stable within 48 h at room temperature. The biodistribution results showed that the ^125I-rMIF was metabolized by the liver, the radioactivity clearance mainly happened in the kidney and the speed of the blood clearance was rapid. After caudal vein injection with ^125I-rMIF, the ratio of radioactivity uptake between inflammatory limb （target） and contra lateral healthy limb （non target） （T/NT） were 1.42, 1.35, 2.18 and 2.05 at 0.5, 1, 6, 24 h respectively. ^125I-rMIF had the capability of locating the inflammatory loci. The advance of it is more obviously at the late stage than that at the early stage. ^125 I-rMIF may be a potential agent for the diagnosis of concealed and subacute inflammatory disease.

关 键 词：重组巨噬细胞移动抑制因子(^125I-rMIF) 炎症 生物学分布 代谢 

分 类 号：TQ463[化学工程—制药化工] R817[医药卫生—影像医学与核医学]