薄层色谱-分光光度法测定纸张中单糖的研究  

Analysis of Monosaccharoses in Paper by Thin Layer Chromatography and Ultraviolet-visible Absorption Spectrometry

作　　者：李继民[1] 王彦吉[2] 邹宁[1] 姚丽娟[1] 

机构地区：[1]中国刑警学院法医系,沈阳110035 [2]中国人民公安大学,北京100038

出　　处：《分析科学学报》2008年第3期270-274,共5页Journal of Analytical Science

基　　金：国家高技术产业发展项目(No.20012492)

摘　　要：建立了薄层色谱-紫外可见分光光度法测定纸张样品水解液中单糖组成的方法。纸张样品水解后点样在以丙酮处理过的硅胶G薄层板上，以正丁醇：乙酸乙酯：异丙醇：乙酸：吡啶：水=7：20：12：7：6：5（体积比）为展开剂，以苯胺-草酸为显色剂，测定了单糖的Rt值。以1-萘酚为显色剂，测定了纸张样品水解液中的单糖含量，方法的线性关系较好，5种单糖的样品加标回收率为96．10％～98．18％。该方法分离效果好，操作简便，可用于纸张样品水解液中5种单糖的同时测定，从而为法庭科学中纸张的检验提供依据。The method for simultaneous determination of five monosaccharoses in the hydrolytic solution of paper by thin layer chromatography and ultraviolet-visible absorption spectrometry was developed. The mixture of n butyl alcohol, ethylacetate, iso-butyl alcohol, acetic acid, pyridine and water （7：20：12：7： 6：5, V/V） was used as developing agent on the TLCS silica gel plate for development. The hydrolytic solution of paper was spotted on the TLCS plate which was dealed with acetone. The Rf values of monosaccharose was determined using aniline-oxalic acid a color reagent. The quantitative analysis was performed by ultraviolet visible absorption spectrometry with the use of naphthol as color reagent. Good linearities were obtained for Xylose, Arabinose, Mannose, Glucose and Galactose. The recoveries of the five monosaccharose for the spiked sample were 96. 10%-98. 18%. The developed method has the advantages of high precision, high sensitivity and simple pretreatment. The method could be applicable to detect the five monosaccharoses in the he hydrolytic solution of copy paper for forensic science.

关 键 词：单糖 纸张 薄层色谱法 紫外可见分光光度法 

分 类 号：O658.1[理学—分析化学]