肽脂质在毛细管胶束电动色谱中的应用  

The Application of Peptide Lipid(N^+C_5Gly2C_(16)) in Micellal Electrokinetic Chromatography

作　　者：蒋本国[1] 朱贵杰[2] 郑华星[1] 张维冰[2] 范圣第[1] 

机构地区：[1]大连民族学院,生物技术与资源利用国家民委-教育部重点实验室,辽宁大连116600 [2]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《分析科学学报》2008年第3期283-286,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20472013)

摘　　要：以自主合成的肽脂质(N+C5Gly2C16)作为表面活性剂进行毛细管胶束电动色谱(MEKC)研究。测定了不同pH值条件下的迁移时间窗口,并且与十六烷基三甲基溴化铵(CTAB)为准固定相的MEKC模式对比,考察了硫脲的保留行为。结果表明,肽脂质可以在较低的浓度下形成胶束,具有背景较低的特征。在优化条件下,采用此系统成功分离了石芽茶提取物,说明以肽脂质作为表面活性剂的MEKC系统具有特殊的分离选择性。The Peptide Lipid （N^＋ C5 Gly2C16 ） synthesized by ourselves was used as surfactants in micellar electrokinetic chromatography. The migration time windows under different pH values were measured and the comparison had been made between peptide lipid and CTAB. The retention behaviors of thiourea under different concentration of peptide lipid has been investigated. The result showed that the micelle of peptide lipid can be formed under low concentration with low background noise. Under the optimized conditions the extracts of shiyacha leaves were separated successfully, which demonstrated that the MEKC with peptide lipid had specific separation selectivity.

关 键 词：肽脂质 毛细管胶束电动色谱(MEKC) 表面活性剂 

分 类 号：O657.8[理学—分析化学]