枯草芽孢杆菌生产胞苷的途径分析  

Metabolic Pathway of Cytidine Synthesis in Bacillus Subtillis

作　　者：魏志强[1] 徐庆阳[1] 刘淑云[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《现代食品科技》2008年第6期544-547,共4页Modern Food Science and Technology

摘　　要：运用METATOOL软件对枯草芽孢杆菌（Bacillus subtillis）TZMl012由葡萄糖生物合成胞苷的代谢途径进行分析，以确定胞苷合成的最佳途径和最大理论产率。结果表明其最大理论产率为0．28，5-磷酸核糖焦磷酸与草酰乙酸是胞苷合成途径的关键节点，发酵中后期低溶氧控制是胞苷合成的重要前提，溶氧控制在5～10％时胞苷产量可提高15．8％。结论是以途径分析为指导，改变外界环境因子，胞苷的产量可得到显著的提高。METATOOL soft ware was applied to analyze the cytidine biosynthesis pathway in Bacillus subtillis TZMI012. The results was as follows： PRPP and oxaloacetic acid were found to be the key nodes in cytidine biosynthesis pathway and low dissolved oxygen concentration in the medium-term and last periods of fermentation was an important precondition of cytidine biosynthesis. The highest theoretical yield of cytidine was 0.28 and cytidme yield could increased by 15.8% if the dissolved oxygen concentration in fermentation broth was of 5%-10%. According to the analysis of metabolic pathway of cytidine biosynthesis, the yield of cytidine can be greatly improved by changing the environmental factors.

关 键 词：胞苷 途径分析 枯草芽孢杆菌 

分 类 号：TQ464[化学工程—制药化工]