硒酸精氨酸对D-gal衰老小鼠学习记忆能力的影响  

Effect of Selenoarginine on Learning and Memory Abilities of D-gal Aging Mice

作　　者：马艳弘[1] 刘安军[1] 张国蓉[1] 朱振元[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《中北大学学报（自然科学版）》2008年第3期258-264,共7页Journal of North University of China(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20576101)

摘　　要：采用颈背部皮下注射大剂量D-半乳糖(D-gal)造模,小鼠分为正常组、模型组、两个剂量的硒酸精氨酸组(8.35μg Se/kg.d和16.78μg Se/kg.d),硒酸精氨酸以灌胃方式给药42 d,Morris水迷宫(Morris Water Maze,MWM)测定小鼠学习记忆能力,称重法计算脑指数,生化分析法测定小鼠全脑总蛋白和脑内乙酰胆碱酯酶(AchE)活力.Morris水迷宫试验结果表明,与模型组相比,硒酸精氨酸组能显著缩短小鼠的逃逸潜伏期(P<0.01),明显增加小鼠穿越平台次数和在平台象限的游泳路程比(P<0.01),其搜索方式主要以趋向式和随机式为主.脑指数测定和生化分析结果表明,与模型组相比,两个剂量硒酸精氨酸组的脑指数、脑总蛋白含量显著提高(P<0.01),乙酰胆碱酯酶活力明显下降(P<0.05 or P<0.01).硒酸精氨酸对D-gal衰老小鼠空间学习记忆能力有明显的改善作用,其作用机制与其能提高脑内谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)等抗氧化物酶活力,增强脑内自由基清除能力,提高抗氧化防御机制有关.Aging model was induced by s.c. injection D-galactose. Mice were divided into normal group, model group and two selenoarginine groups（8.35 μg Se/kg·d and 16.78μg Se/kg · d respectively）. The selenoarginine was administrated by i.g. method for 42 days. The Morris water maze （MWM） test was used to observe learning and memory abilities of mice. Biochemistry method was used to determine the activities of acetylcholinesterase （AchE） and the protein contents in the brain tissue and brain index were also measured. Results of Morris water maze test show that, after model mice are treated by selenoarginine, the escape latency for finding the hiding platform is significantly shortened compared with the model mice（P〈0. 01）; on the other hand , the total crossing numbers at the previous platform and the mean percentages of swimming distance in total swim patch located in the platform quadrant of pool are increased obviously compared with the model mice（P〈0.01）. The swimming search strategies are mainly taxis or random in two selenoarginine groups mice. Biochemistry method shows that, among the seleno-argnine mice, brain index, protein contents and Ache activities in the brain tissue are significant- ly increased compared with the model mice （P〈0.01）. Selenoarginine is involved in improvement of learning and memory abilities of D-gal aging mice. The protective mechanism of selenoarginine can be attributed to the elevation of the activity of antioxidase and enhanced antioxidant defenses.

关 键 词：硒酸精氨酸 水迷宫 脑蛋白 乙酰胆碱酯酶 

分 类 号：R338[医药卫生—人体生理学] Q581[医药卫生—基础医学]