HBV感染的不同血清学指标组合的HBV-DNA检测  

Positive detection for HBV-DNA of different serological markers combination in patients with HBV infection

作　　者：王建东[1] 赵凤桐[1] 崔吉[1] 夏薇[2] 

机构地区：[1]吉林市中心医院,吉林吉林市132011 [2]北华大学医学部

出　　处：《中国实验诊断学》2008年第6期731-732,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的了解HBV感染的不同血清学指标组合的HBV-DNA阳性情况。方法用荧光定量聚合酶链反应和酶联免疫吸附试验(ELISA)分别检测1531份乙型肝炎患者和健康体检者血清的HBV-DNA和HBV血清标志物(HBV-M)。结果1组(HBsAg、HBeAg、抗HBc阳性)、2组(HBsAg、抗HBe、抗HBc阳性)、3组HBsAg阳性的患者HBV-DNA的阳性率分别为99.5%、57.1%、15.4%,其拷贝数分别为1.14×108/ml,1.43×106/ml,6.64×104/ml。结论荧光定量PCR对乙型肝炎早期诊断、传染性的判断及疗效观察具有临床实用及指导意义。Objective To investigate the positive HBV-DNA of different serological markers combination in patients with HBV infection.Methods HBV-DNA and HBV serum markers in 1531 patients of hepatitis B and healthy peoples were detected by fluorescent quantitative PCRand ELISA.Results The positive rate of Groupl（HBsAg＋ ,HBeAg＋ and HBcAb＋ ） was99.5%.Theposi- tive rate of Group2（HBsAg ＋ ,HbeAb ＋ and HBcAb＋ ） was 57.1% and the positive rate of Group3（HBsAg ＋ ）was 13.0%.Their copy counts were 1.14 ×10^8, 1.43 × 10^6 and 6.64 × 10^4 ml^- 1 respectively. Conclusion Fluorescent quantitative PCR has applicative and guidance significance in early diagnosis and infectivity judgment and therapeutic effect assess.

关 键 词：荧光定量聚合酶链反应 HBV-DNA 乙型肝炎病毒标志物 

分 类 号：R512.6[医药卫生—内科学] R450[医药卫生—临床医学]