转harpin_(Xooc)蛋白编码基因hrf2对油菜抗菌核病的影响  

Transgenic Rape with hrf2 Gene Encoding harpin_(Xooc) Resistant to Sclerotinia Sclerotinorium

作　　者：马玲莉[1] 霍蓉[1] 高学文[1] 何丹[1] 邵敏[1] 王琦[1] 

机构地区：[1]南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室,南京210095

出　　处：《中国农业科学》2008年第6期1655-1660,共6页Scientia Agricultura Sinica

基　　金：国家高技术研究发展计划(863计划)(2006AA10Z172);国家植物转基因专项(JY03B1202)

摘　　要：【目的】尝试利用转基因方法提高油菜抗病性。【方法】将来自水稻细菌性条斑病菌(Xanthomonas oryzae pv.oryzicola)编码harpinXooc蛋白的hrf2基因插入植物表达载体pCAMBIA1301中,构建了植物重组表达质粒pCAMBIA1301-hrf2。由根癌农杆菌LBA4404介导,将重组表达质粒pCAMBIA1301-hrf2转化甘蓝型油菜杨油4号子叶节。【结果】获得了抗潮霉素的再生转基因油菜植株,经PCR,RT-PCR和GUS染色检测证明hrf2基因已整合到油菜基因组中。抗病性鉴定结果表明,转基因油菜对油菜菌核病有较好的抗性,在转基因植株叶片上油菜菌核病菌菌丝的生长受到明显抑制。【结论】利用农杆菌转化法可将hrf2基因导入油菜基因组中,并使转基因油菜对菌核病的抗性提高。【Objective】The objective of this study was to improve the resistance of rape using the method of transformation. 【Method】The gene hrf2 derived from Xathomonas oryzae pv. oryzicola encoding harpinXooc protein was inserted into transgenic vector pCAMBIA1301. The cotyledonal petiole segments from rapeseed variety Yangyou 4 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA1301-hrf2. 【Result】Hygromycin-resistant green shoots were obtained. PCR,RT-PCR and GUS analyses have confirmed successful integration of the foreign gene into the genome of the rapeseed variety Yangyou 4. Disease bioassays of transgenic plants revealed that the transgenic plants with resistance to Rape sclerotiniose was obtained. 【Conclusion】 The hrf2 gene can be transferred into rape using the method of Agrobacterium-mediated transformation,which improved the resistance to S. sclerotinorium in the transgenic plant.

关 键 词：hrf2基因 农杆菌介导转化法 转基因油菜 抗病性 

分 类 号：S565.4[农业科学—作物学] Q753[生物学—分子生物学]