应用微卫星DNA技术研究中华蜜蜂群内工蜂监督效果  

Study on the Worker Policing in Apis Cerana Cerana Based on Microsatellite DNA

作　　者：谢宪兵[1] 苏松坤[2] 郑云林[1] 吴小波[1] 曾志将[1] 

机构地区：[1]江西农业大学蜜蜂研究所 [2]浙江大学动物科学院,杭州310029

出　　处：《中国农业科学》2008年第6期1816-1821,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30560114);泉州师范学院校自选项目(2007KJ004)

摘　　要：【目的】研究不同中华蜜蜂(Apis cerana cerana)群内的工蜂繁殖现象,探讨蜂群的工蜂监督效果。【方法】以中华蜜蜂为试验材料,对处女蜂王分别进行单雄和双雄人工授精,并以蜂王自然交尾的蜂群作为对照。蜂王成功繁殖后7周,利用蜜蜂微卫星DNA技术检测蜂群内的雄蜂是由蜂王产的未受精卵发育而成,还是由工蜂产的未受精卵发育而成。【结果】所有蜂群中的雄蜂都是由蜂王产的未受精卵发育而成。【结论】在人工授精和自然交尾蜂群中都明显存在工蜂监督行为。【Objective】For exploring the effect of worker policing in Apis cerana cerana,the worker reproduction was studied in different colonies. 【Method】 Biology of the Apis cerana cerana was the studied subject in this paper. Virgin queens were instrumentally inseminated with 1 or 2 males,and the controlled ones were naturally mated. Seven weeks later,the queens reproduced successfully,drones were detected whether developed from the unfertilized eggs laid by queen or by workers,which based on the technology of honeybee microsatellite DNA that sampled the pupae of worker and drone in each colony. 【Result】 The drones were developed from the unfertilized eggs laid by queen in all of the colonies. 【Conclusion】 The activities of worker policing are taking place universally in colonies that the queens are instrumentally inseminated and naturally mated.

关 键 词：中华蜜蜂 工蜂繁殖 工蜂监督 微卫星DNA 人工授精 

分 类 号：S891[农业科学—特种经济动物饲养]