肉种鸡MDV和REV人工共感染的动态病理学与抗原定位研究  

Dynamic Pathology and Antigen Location Study on Broiler Breeders with Coinfection of MDV and REV

作　　者：刁秀国[1] 张利[1] 成子强[1] 朱国[1] 王桂花[1] 孟祥凯[1] 高婷婷[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国农业科学》2008年第6期1838-1844,共7页Scientia Agricultura Sinica

基　　金：中国博士后科学基金项目;中国博士后科学基金二等奖资助金(2005038258)

摘　　要：【目的】深入了解MDV与REV人工共感染肉种鸡后疾病的发生、发展状况,为二者临床复杂的混合感染提供确实的鉴别诊断方法和明确的诊断时间。【方法】MDV和REV强毒株人工共感染1日龄肉种鸡,定期剖检,进行病理组织学、细胞凋亡、免疫组化和肿瘤组织的超微结构观察。【结果】肝、胰腺、腺胃、盲肠扁桃体、心肌在感染后1周出现以小淋巴细胞浸润为主的炎症,2～9周时,大部分实质器官出现以幼稚淋巴细胞、大中小淋巴细胞为主的渐进性增生灶,部分增生灶中可见原始网状细胞和马立克氏病细胞;10周后免疫器官实质细胞出现明显的凋亡;免疫组化显示,肝、脾、法氏囊、胸腺等在2周时呈双抗原阳性;电镜下,肝脏肿瘤组织中可见多形态淋巴细胞,核分裂相和细胞凋亡同时存在,同一细胞中可见MDV和REV两种病毒粒子。【结论】MDV与REV共感染对病程起协同和促进作用,发病早,病变明显;免疫酶及荧光方法可用于共感染的早期诊断(2周以前);而后期(4周以后)可通过病理组织学进行鉴别诊断,两种病毒粒子、马立克氏病细胞、原始网状细胞、多形态和幼稚淋巴细胞可作为诊断依据。【Objective】To further understand the generation and development of coinfection of MDV and REV in broiler breeders,then find the method and the optimal time of differential diagnosis for complex clinic multiple infection.【Method】 Pathohistological changes,apoptosis,immunohistochemistry and ultrastructure of tumor issues of broiler breeders inoculated with MDV and REV were studied. 【Result】The study showed that proliferation of small lymphocytes could be seen in main organs at 1 week old,then immature lymphocytes,all kinds of lymphocytes,primitive reticular cells and Marek’s disease cells were observed in 2-9 weeks. Cell apoptosis couldn’t be seen untill 10 week in immune system. Immunohistochemistry detection showed that positive sign of MDV and REV antigen were observed in main organs at 2 weeks old. Multi-morphology lymphocytes,MDV and REV,mitotic figures and apoptosis of lymphocytes were observed with transmission electron microscope.【Conclusion】MDV cooperating with REV promote the course of disease of coinfection. Pathological changes of coinfection come on earlier and more severe than single infection of MDV and REV. Differential diagnosis can be done by immunohistochemistry in early stage（before 2 weeks）,and pathohistology in late stage （post 4 weeks）. Marek’s disease cells,primitive reticular cells,immature lymphocytes and two viruses can serve as a basis for pathohistology differential diagnosis.

关 键 词：马立克氏病病毒 网状内皮组织增殖病病毒 共感染 动态病理学 抗原定位 肉种鸡 

分 类 号：S858.31[农业科学—临床兽医学]