中国荷斯坦牛STAT5A基因遗传多态性与泌乳性状的相关分析  

Polymorphisms of STAT5A Gene and Its Association with Milk Performance Traits in Chinese Holstein Cattle

作　　者：鲍斌[1] 房兴堂[1] 陈宏[1] 张润锋[2] 严林俊[3] 张海军[1] 

机构地区：[1]徐州师范大学细胞与分子生物学研究所,江苏徐州221116 [2]西北农林科技大学动物科技学院/陕西省农业分子生物学重点实验室,陕西杨凌712100 [3]南通农业职业技术学院,江苏南通226007

出　　处：《中国农业科学》2008年第6期1872-1878,共7页Scientia Agricultura Sinica

基　　金：国家“863”计划项目(2006AA10Z197);陕西省自然科学基金(2004C122);徐州师范大学重点人才基金(XY200234);西北农林科技大学拔尖人才支持计划资助

摘　　要：【目的】研究STAT5A基因多态性与中国荷斯坦牛泌乳性状的相关性,为进一步加快育种进程提供参考依据。【方法】利用PCR-SSCP技术对279头中国荷斯坦牛的STAT5A基因的P1和P2两个位点进行群体遗传学检测;计算其基因频率和基因型频率,并对多态性与泌乳性状进行相关性分析。【结果】发现在STAT5AP1和STAT5AP2两个位点上分别存在3种多态以及7种单倍型组合。STAT5AP1位点的AA、GG和AG的基因型频率分别为0.2401、0.1470和0.6129,A和G的基因频率分别为0.5466和0.4534,不同基因型对第1胎产奶量、乳蛋白率和第2胎乳蛋白率存在显著影响;STAT5AP2位点的CC、TT和CT的基因型频率分别为0.7527、0.0036和0.2437,C和T的基因频率分别为0.8746和0.1254,不同基因型对第2胎乳蛋白率有显著影响;7种单倍型组合AACC、AGCC、AACT、AGCT、GGCT、GGCC和AATT的频率分别为0.1864、0.4624、0.0502、0.1505、0.0430、0.1039和0.0036,不同单倍型与第1和2胎产奶量以及乳蛋白率存在相关性。【结论】STAT5A基因不同基因型和单倍型组合与中国荷斯坦牛产奶量和乳蛋白率存在相关性,但与乳脂率不相关。【Objective】The polymorphisms of STAT5A gene and their correlation to milk performance traits in Chinese Holstein cattle were investigated for the purpose of providing molecular maker information to facilitate the breeding efficiency.【Method】 PCR-SSCP was applied to analyze the polymorphisms of two loci in STAT5A gene in 279 Chinese Holstein cattle. Genotypic frequencies,allelic frequencies,correlation analysis between the polymorphisms and milk performance traits were estimated. 【Result】Three genotypes and seven haplotype combinations were found at the two loci. The frequencies of genotypes AA/GG/AG were 0.2401/0.1470/0.6129 and those of alleles A/G were 0.5466/0.4534 at locus P1. The result showed that the genotypes had significant effect on milk yield in the first lactation and protein rate in both the first and second lactations. Genotypic frequencies of CC/TT/CT were 0.2401/0.1470/0.6129 and allelic frequencies of C/T were 0.5466/0.4534 at locus P2. Different genotypes had remarkable influence on protein rate in the second lactation. In addition,the frequencies of seven haplotype combinations were 0.1864,0.4624,0.0502,0.1505,0.0430,0.1039,and 0.0036,and different haplotype combinations affected the milk yield and protein rate in the first and second lactations.【Conclusion】 The correlation was detected between different genotypes and haplotype combinations and milk yield and protein rate at these two loci,but there was no correlation between the polymorphisms and milk fat rate.

关 键 词：STAT5A PCR-SSCP 中国荷斯坦牛 多态性 单倍型 

分 类 号：S823[农业科学—畜牧学]