氟西汀对慢性应激大鼠血清皮质醇、肿瘤坏死因子-α、胃黏膜细胞间黏附分子-1表达的影响  

Effects of fluoxetine on serum cortisol, TNF-α and expression of ICAM-1 in gastric mucosa in chronic stress rats

作　　者：夏静[1] 邵云[1] 李艳辉[1] 王旭梅[1] 金魁和[2] 

机构地区：[1]中国医科大学附属盛京医院心理科,沈阳110004 [2]中国医科大学

出　　处：《中国行为医学科学》2008年第6期525-527,共3页Chinese Journal of Behavioral Medical Science

摘　　要：目的研究氟西汀对慢性应激大鼠血清皮质醇、肿瘤坏死因子-α（TNF—α）含量、胃黏膜细胞间黏附分子-1（ICAM-1）表达的影响。方法将青年雄性Wistar大鼠24只随机分为对照组、应激组、氟西汀组各8只。应激组和氟西汀组大鼠每笼1只喂养，实验第1～21天，接受各种不同的应激。氟西汀组大鼠每天给予氟西汀水溶液灌胃。对照组大鼠群养不给任何刺激。实验第22天杀死所有大鼠，检测血清皮质醇、TNF—α浓度；用免疫组化法检测胃黏膜蛋白ICAM—1表达，进行图像分析，测定光密度平均值。结果应激组大鼠与对照组比较，血清皮质醇含量[（77．12±9．76）μg/ml，（44．96±6．25）μg／ml，t=7．85，P〈0．01]、TNF—α含量[（69．66±6．68）pg／ml，（39．21±3．57）pg／ml，t=11．37，P〈0．01]、胃黏膜ICAM—1表达的光密度平均值[（53．87±6．84），（30．26±3．68），t=8．59，P〈0．01]有明显增高；与应激组比较，氟西汀组大鼠血清皮质醇含量[（58．82±6．56）μg／ml，t=4．40，P〈0．01]、TNF—α含量[（50．18±3．23）pg／ml，t=7．43，P〈0．01]、胃黏膜ICAM—1表达的光密度平均值（36．61±4．39，t=6．00，P〈0．01）明显下降。结论慢性应激可引起大鼠血清皮质醇、TNF—α含量增高，胃黏膜ICAM-1过表达，而氟西汀可以部分的逆转这些改变。Objective To study the effects of fluoxetine on serum cortisol, TNF-α and expression of ICAM-1 in gastric mucosa in chronic stress rats. Methods Male Wistar rats were divided into control group, stress group and fluoxetine group randomly, and 8 rats each group. Stress group and fluoxetine group were separated one rat in each box, from 1 - 21 days, and accepted various types of stresses. At the same time, fluoxetine group were given fluoxetine every day. The control group were fed in two boxes with no stress from 1 - 21 days. On 22nd day, all the rats were killed. The concentrations of cortisol and TNF-α in serum were measured. Immunohistochemistry method was used to measure the expression of ICAM-1 protein in gastric mucosa and analyze optical density （OD） average of ICAM-1. Results After 21 days stress,compared with control group, cortisol [ （77.12 ±9.76） μg/ml, （44.96 ± 6.25 ） μg/ml, t = 7.85, P 〈 0.01 ], TNF-α [ （ 69.66±6.68 ） pg/ml, （ 39.21 ± 3.57 ） pg/ml, t = 11. 37, P〈0. 001 ]concentration and OD of ICAM-1 （53.87 ±6.84,30.26 ± 3.68, t= 8.59, P〈0.01 ）inereased significantly in stress group. Compared with stress group, cortisol [ （58.82 ± 6.56）μg/ml, t = 4.40, P 〈 0.01 ], TNF-α （50.18 ±3.23） pg/ml, t =7.43, P〈0.01 ]concentration and OD of ICAM-1 （36.61 ±4.39, t=6.00, P 〈 0.01 ） decreased in fluoxetine group. Conclusion Chronic stress result in serum cortisol ,TNF-α increase and overexpression of ICAM-1 in gastric mucosa. Those changes can be reversed by fluoxetine to some extent.

关 键 词：氟西汀 慢性应激 皮质醇 肿瘤坏死因子-Α 细胞间黏附分子-1 胃黏膜 

分 类 号：R9[医药卫生—药学]