24个白桑(Morus alba L．)地方品种的遗传多样性分析  

ISSR Analysis of Genetic Diversity on 24 Local Varieties of Morus alba Linne

作　　者：黄勇[1] 张林[1,2,3] 赵卫国[1,2] 沈兴家[1,2] 潘一乐[1,2] 

机构地区：[1]江苏科技大学生物与环境工程学院,江苏镇江212018 [2]中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,江苏镇江212018 [3]南京农业大学生命科学学院,南京210095

出　　处：《蚕业科学》2008年第2期302-306,共5页ACTA SERICOLOGICA SINICA

基　　金：国家科技基础条件平台工作子项目(编号2005DKA21002-09)

摘　　要：采用ISSR分子标记技术对山东、河北地区的24个白桑（Morus alba L．）地方品种资源进行了遗传多态性分析。筛选的13条ISSR引物共扩增86条扩增带，其中多态性条带63条，多态性比率为73．25％，ISSR标记遗传相似系数范围在0．6706～0．9529。通过类平均聚类（UPGMA）法分析，24份材料聚分为2大类。Inter-simple sequence repeat （ISSR） molecular marker was used to analyze the genetic diversity of 24 local varieties of Morus alba Linne from Shandong and Hebei province in this paper. By using 13 selected ISSR primers, 86 DNA bands were amplified, in which 63 bands showed polymorphism with a rate of 73. 25%. The coefficient ranges of genetic similarity of the ISSR were 0, 670 6 -0. 952 9. Twenty-four local va- rieties were classified into 2 groups by UPGMA method. The above results suggested that ISSR markers can be effectively used to evaluate the genetic diversity of local varieties of Morus alba Linne, and provided the molecular foundation for the systematic research.

关 键 词：白桑种 地方品种 简单重复序列间扩增 遗传多样性 

分 类 号：S888.3[农业科学—特种经济动物饲养]