流感病毒裂解方法优化研究  

Viral lysis of influenza virus vaccine

作　　者：魏晓露[1] 高菁霞[1] 何香莲[1] 黄秋香[1] 姚石宝[1] 郭自全[1] 唐成丽[1] 廖国阳[1] 李映波[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,云南昆明650118

出　　处：《重庆医学》2008年第12期1307-1308,1311,共3页Chongqing medicine

基　　金：国家863资助课题(2006AA02Z409);云南省自然科学基金资助项目(2004C0720M;2006XY29)

摘　　要：目的研究流感病毒的裂解剂及裂解灭活条件。方法使用两种裂解剂TritonX100和TritonN101以不同浓度对流感病毒单价纯化液作用，通过电镜进行裂解效果观察，确定最佳裂解时间，用单向免疫扩散法检测HA含量作为评价指标。同时，观察先灭活再裂解和先裂解再灭活对病毒裂解效果的影响。结果TritonX100的裂解效果较优越，0．5％～1％TritonX100作用90min可使流感病毒裂解完全；TritonX100与甲醛作用的先后顺序对病毒的裂解灭活效果影响不大。结论0．5％～1％TritonX100可得到流感病毒的最佳效果。Objective To determine the suitable lytic agent as well as lysis and inactivation procedure for influenza virus. Methods The two kinds of surface active agents Triton X100 and TritonN101 were used to lyse influenza virus with different concentration,and the lysates were observed under electron microscope for determining the optimal time. The HA content was measured by single radial immunodiffusion assay. The sequence of inactivation and lysis also was considered as well. Results The optimal procedure was developed by the lysis of influenza virus with 0.5%- 1%Triton X100 for 90min. The sequence of inactivation and lysis with Triton X 100, formaldehyde was not different. Conclusion The optimal procedure is 0.5%- 1%Triton X100.

关 键 词：流感病毒 裂解 

分 类 号：R373[医药卫生—病原生物学]