生长素、细胞分裂素对黑暗和ABA诱导蚕豆气孔关闭的抑制效应  

The Inhibitory Effects of Auxin and Cytokinin on Dark- and ABA-induced Stomatal Closure in Broad Bean

作　　者：张蓓[1] 佘小平[1] 张广斌[1] 孟朝妮[1] 宋喜贵[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《作物学报》2008年第6期1034-1041,共8页Acta Agronomica Sinica

基　　金：陕西省自然科学研究计划项目(2003C101;2005C112)

摘　　要：以蚕豆(Vicia faba L.)为材料,借助表皮条实验和激光扫描共聚焦显微镜技术研究合成和天然生长素、细胞分裂素吲哚乙酸(IAA)、萘乙酸(NAA)、2,4-二氯苯氧乙酸(2,4-D)、玉米素(ZT)、激动素(KT)和6-苄基腺嘌呤(6-BA)对黑暗和脱落酸(ABA)诱导气孔关闭的效应及其机制。结果表明,黑暗和ABA均诱导气孔关闭,与NO清除剂羧基-2-苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物(c-PTIO)、牛血红蛋白(Hb)和NO合酶(NOS)抑制剂NG-N-L-精氨酸甲酯(L-NAME)以及过氧化氢(H2O2)清除剂抗坏血酸(AsA)、过氧化氢酶(CAT)和过氧化氢产生酶NADPH氧化酶抑制剂二苯基碘(DPI)一样,供试合成和天然生长素、细胞分裂素均显著逆转黑暗和ABA诱导的气孔关闭,暗示生长素、细胞分裂素可能通过降低NO和H2O2水平起作用。保卫细胞内源NO和H2O2检测结果显示,合成和天然生长素、细胞分裂素确实均显著降低暗和ABA诱导的NO和H2O2水平。考虑到IAA、ZT分别为天然生长素、细胞分裂素的典型代表而且供试生长素、细胞分裂素均包括了合成和天然种类,可以认为供试生长素、细胞分裂素逆转暗和ABA诱导的气孔关闭及降低NO、H2O2水平的作用可能是所有生长素类、细胞分裂素类植物生长物质均具备的效应。In the present studies, the effects and mechanisms of natural and synthetic auxin IAA, NAA, 2,4-D, cytokinin ZT, KT, 6-BA on dark- and ABA-induced stomatal closure were investigated by means of stomatal bioassay and using laser-scanning confocal microscopy. Isolated epidermal strips of Viciafaba were incubated with IAA （10 μmol L^-1）, NAA （10μmol L^-1）, 2,4-D （10 μmol L^-1）, ZT （0.1 μmol L^-1）, KT （0.2 μmol L^-1）, 6-BA （0.2 μmol L^-1）, NO scavenger c-PTIO （200 μmol L^-1）, Hb （100 μmol L^-1）, NOS inhibitor L-NAME （25 μmol L^-1）, H2O2 scavenger AsA （100 μmol L^-1）, CAT （100 U mL^-1）, inhibitor of HEOE-generating enzyme NADPH oxidase DPI （10 μmol L^-1 ） for 3 h, in darkness or in light in the presence of ABA （1 μmol L^-1）, respectively. The results showed that auxin, cytokinin, as well as c-PTIO, Hb, L-NAME, AsA, CAT, and DPI, reversed dark- and ABA-induced stomatal closure significantly. Epidermal strips treated with auxin and cytokinin were loaded with NO-fluorescent dye DAF-2DA or HEOE-fluorescent dye HEDCF-DA. The results indicated that darkness and ABA could induce an intense DAF-2DA or H2DCF-DA fluorescence in guard cells. However, dark- and ABA-induced DAF-2DA and HEDCF-DA fluorescence were largely prevented by auxin and cytokinin tested. Similarly, the treatments of c-PTIO, Hb, L-NAME and AsA, CAT, DPI also substantially suppressed dark- and ABA-induced DAF-2DA and H2DCF-DA fluorescence, respectively. These results provide the evidence that auxin and cytokinin tested lessen assuredly NO and H2O2 levels induced by dark and ABA in guard cells. Considering synthetic auxin, cytokinin NAA, 2,4-D, KT, 6-BA and natural IAA, ZT were used in the present work and IAA, ZT are representative of endogenous auxin and cytokinin respectively, the effects of auxin, cytokinin tested on dark- and ABA-induced stomatal closure and NO, H2O2 level can be attributed to an universal effect of auxin or cytokinin.

关 键 词：生长素 细胞分裂素 一氧化氮 过氧化氢 暗 脱落酸 气孔关闭 

分 类 号：S643.6[农业科学—蔬菜学]