棉花4个GL2同源基因的序列比较及表达分析  

Sequence Analysis and Characterization of Four GL2 Homologous Genes in Cotton (Gossypium hirsutum L.)

作　　者：杨霞[1] 侯磊[1] 肖月华[1] 李德谋[1] 张国芳[1] 裴炎[1] 

机构地区：[1]西南大学生物技术中心/农业部生物技术与品质改良重点开放实验室,重庆400715

出　　处：《作物学报》2008年第6期1086-1091,共6页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2004CB117304)

摘　　要：拟南芥GLABRA2(AtGL2)在决定拟南芥叶毛和根毛细胞的分化中起重要作用。通过同源序列查询从棉花的EST库中找到4个与AtGL2高度同源的全长编码序列(GhHOX1、GhHOX2、GhHOX3和GhHOX4)。序列分析表明4个棉花HOX基因与AtGL2基因Homeobox结构域相同氨基酸分别达到95%、71%、72%和63%。实时RT-PCR分析结果显示,GhHOX1、GhHOX3和GhHOX4均在伸长期的纤维中优势表达;而GhHOX3在开花当天野生型胚珠中的表达量明显高于无绒无絮突变体。说明棉花GL2同源基因在棉花纤维的起始和伸长中具有重要的调控作用。Arabidopsis GLABRA2 （AtGL2）, a transcription factor, plays critical roles in the differentiation of trichome cells and root hair cells. However, the function of GL2 homologous genes in cotton fiber differentiation and development is still unclear. In this paper, transcription profiles of four putative cotton HOX ORFs homologous with AtGL2 were investigated. Multiple sequence alignment revealed that these 4 GhHOX proteins shared 95%, 71%, 72%, and 63% identity with AtGL2 in the homeobox conserved domain, respectively. Real-time RT-PCR analysis indicated that GhHOX1, GhHOX3 and GhHOX4 expressed in cotton elongating fiber cells preferentially. Moreover, GhHOX3 showed significant higher expression level in the ovules of Xuzhou 142 than in its fuzz-free and lint-free mutant ovules at the initiation stage. The results suggested that the GL2 homologous genes of cotton may play important roles in fiber initiation and elongation.

关 键 词：棉花 GL2同源基因 Real-TimeRT-PCR 

分 类 号：S562[农业科学—作物学] Q78[生物学—分子生物学]