EB病毒感染患儿细胞因子水平与T细胞亚群检测的临床意义  

CLINICAL SIGNIFICANCE OF THE CYTOKINE LEVEL OF CHILDREN INFECTED WITH EB VIRUS AND DETECTION OF T CELL SUBGROUP

作　　者：叶中绿[1] 庞伟君[2] 黄秀兰[1] 

机构地区：[1]广东医学院附属医院儿科,湛江524001 [2]广东医学院附属医院儿科检验科

出　　处：《现代预防医学》2008年第12期2306-2308,共3页Modern Preventive Medicine

摘　　要：[目的]探讨EB病毒感染后患儿免疫功能变化及对临床预后的影响。[方法]EB病毒感染后患儿30例,正常对照儿童20例。分别用双抗体夹心酶联免疫吸附法(ELISA法)检测血清中的白介素-6(IL-6)、白介素-8(IL-8)水平,流式细胞术检测各T细胞亚群变化。[结果]EB病毒感染后患儿CD3+、CD4+、CD4+/CD8+T细胞明显低于正常对照组(P﹤0.01)和治疗后组,而CD8+、IL-6、IL-8明显高于正常组(P﹤0.01或0.05)和治疗后组。[结论]EB病毒感染后患儿免疫性细胞因子分泌紊乱,细胞免疫功能受抑制。[Objective] To explore the change of immune fimction of children infected with EB virus and its significamt to clinical prognosis. [Methods] 30 children infected with EB were recruited as experimental group, and 20 children were collected as controls. The levels of IL-6 and IL-8 were determined with ELISA method and the changes of T cell subgroups were detected by flow cytometry. [ Results] The number f CD3^＋, CD4^＋and CD4^＋/CD8^＋ T cells among children infected with EB virus were significantly lower than those among controls and children after treatment （P 〈 0.01 ）, while the number of CD8^＋ and the levels of IL-6 and IL-8 among experimental group were significantly higher than those among the control group and children after treatment. [Conclusion] The parasecretion of immune cytokine were occurred in children infected with EB virus, and the cellular immune function was also inhibited.

关 键 词：EB病毒 免疫调节 细胞因子 T细胞亚群 流式细胞术 

分 类 号：R373[医药卫生—病原生物学]