人类嗜T淋巴细胞病毒I型的PCR检测方法研究  

STUDY ON THE PCR FOR THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE I DETECTED BY PCR

作　　者：白松涛 魏天佐 

机构地区：[1]深圳市龙岗区血站,深圳518116 [2]深圳市龙岗区葵涌街道预防保健所

出　　处：《现代预防医学》2008年第12期2312-2313,共2页Modern Preventive Medicine

摘　　要：[目的]建立人类嗜T淋巴细胞病毒I型(HTLV-I)PCR检测方法。[方法]采集成人T淋巴细胞白血病(ALT)病人全血,分离外周血单核细胞(PBMCs),并与健康人PBMCs共培养分离HTLV-I;针对HTLV-I的gag和tax基因设计引物,进行PCR扩增,对扩增产物进行琼脂糖凝胶电泳检测HTLV-I。[结果]凝胶电泳检测到HTLV-I前病毒DNA的gag和tax片断。[结论]PCR法可快速、准确检测嗜人T细胞白血病病毒。[Objective] To establish the polymerase chain reaction （PCR） method for detection of Human T-cell Lymphotropic virus type I （HTLV-I）. [Methods] PBMCs from peripheral blood specimens of patients with ATL were collected and cultured together With peripheral blood monocytes （PBMCs） donated by uninfected healthy donors to isolate HTLV-I; The primers regarding gag and tax of HTLV-I were designed to amplify with PCR, HTLV-I in the amplified products was detected by agamse gel electrophoresis. The DNA was amplified by PCR using primers gag and tax. The amplified products were detected with agarose gel electrophoresis. [ Results] The gag and tax section in DNA of HTLV-I provirus could detected by agarose gel electrophoresis. [Conclusion] PCR can rapidly and accurately detect the HTLV-I.

关 键 词：人类嗜T淋巴细胞病毒(HTLV-I) 前病毒 PCR 

分 类 号：R446[医药卫生—诊断学] R733.7[医药卫生—临床医学]