HPLC法测定复方软甲膏中水杨酸和盐酸丁卡因的含量  

Determination of salicylic acid and tetracation hydrochloride in Compound onychomycosis cream by HPLC

作　　者：顾伟锋[1] 朴淑娟[1] 张纯[1] 

机构地区：[1]第二军医大学长征医院药学部,上海200003

出　　处：《药学实践杂志》2008年第3期217-219,共3页Journal of Pharmaceutical Practice

摘　　要：目的：建立测定复方软甲膏中水杨酸和盐酸丁卡因含量的HPLC法。方法：色谱柱为Agilent-C18（4．6mm×150mm，5μm）；流动相为甲醇-水-磷酸（50：50：0．04），流速1．0mL／min，检测波长313nm。结果：水杨酸溶液在82．00～410．00μg／mL时与其吸收值呈良好线性关系（n=0．9999），平均加样回收率99．80％，RSD=1．68％；盐酸丁卡因溶液在5．00～25．00μg／mL时与其吸收值呈良好线性关系（n=0．9999），平均加样回收率99．75％，RSD=1．36％。结论：本方法操作简便、快捷，结果准确、可靠，适用于复方软甲膏的质量控制。Objective：To establish a HPLC method for the determination of salicylic acid and tetracation hydrochloride in compound onychomycosis cream. Methods： Agilent-C18 （4. 6 mm × 150 mm, 5 μm） column was used with a mobile phase of methanolwater-phosphoric （50 ： 50 ： 0. 04） and a flow rate of 1. 0 m .E/rain, at the detection wavelength of 313 nm. Results：This method showed good linear relation （r = 0. 999 9） in the range of 82. 00 -410. 00 μg/mL for salicylic acid, and 5.00 -25.00 μg/mL for tetracation hydrochloride. The average recoveries were 99. 80% and 99. 75%, with the relative standard deviation of 1.68% and 1.36% , respectively. Conclusion：The method is simple, quick, accurate and suitable for the quality control of compound onychomycosis cream.

关 键 词：高效液相色谱法 水杨酸 盐酸丁卡因 

分 类 号：R917[医药卫生—药物分析学]