锥底生物反应器的动物细胞培养  

Performance of the Frusto-Conical Bottom Bioreactor in Mammalian Cell Suspension Culture

作　　者：白力[1] 张淑香[1] 唐寅[1] 郭美锦[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2008年第3期338-341,共4页Journal of East China University of Science and Technology

基　　金：上海市科委重点项目(064319033);上海市重点学科建设项目资助(B505)

摘　　要：宫颈癌细胞(HELA)、人胚肾细胞(HEK293)和中华仓鼠卵巢细胞(CHO)3种细胞系在用于哺乳动物细胞悬浮培养的锥底生物反应器(FCB)、平底生物反应器(FB)和常规机械搅拌式生物反应器(STR)中进行培养和冷模实验。结果表明:FCB中HELA细胞的最高密度比FB高27%,HEK293和CHO细胞的最高密度分别比STR高38%和43%。FCB的混合时间平均比FB短27%,比STR长35%;kLa比FB和STR分别高83%和97%。Three cell lines of HELA, HEK293 and CHO were cultured in a novel inverted frusto-conical bottom bioreactor （FCB）, flat bottom bioreactor （FB） and conventional mechanism stirred bioreactor （STR）, respectively. Highest cell densities in FCB for HELA,HEK293 and CHO cells were 27% ,38% and 43% higher than that in FB,STR and STR, respectively. Additionally, mixing time and ki.a of three types of bioreactors were determined. Average mixing time of FCB is 27% shorter than that of flat bottom one, while 35% longer than that of STR. Average kLa of FCB is 83% and 97% higher than that of FB and STR, respectively.

关 键 词：生物反应器 动物细胞悬浮培养 混合时间 氧传递系数 

分 类 号：Q813.1[生物学—生物工程]