黄芩中黄芩苷的提取工艺及高效液相色谱分析条件的优化  

Baicalin extraction in radix scutellariae and optimization of analysis by HPLC

作　　者：赵丽华[1] 黄昆[2] 

机构地区：[1]华中科技大学化学系,湖北武汉430074 [2]华中科技大学能源学院,湖北武汉430074

出　　处：《实验技术与管理》2008年第6期54-55,117,共3页Experimental Technology and Management

基　　金：华中科技大学2005年实验研究立项项目(200516)

摘　　要：以黄芩为研究对象，建立并优化了高效液相色谱方法，测定了黄芩苷的含量。实验中设计了正交方法，综合考虑煎煮粒度、煎煮时间、加水量和煎煮次数等因素，对黄芩的煎煮工艺进行研究。结果表明，以上4个因素对黄芩苷的溶出量及浸膏率影响程度依次降低，而粉碎粒度的影响最显著。提出了黄芩的优化煎煮工艺参数，在此基础上，得到黄芩最佳粉碎粒度范围。Radix Scutellariae was chosen as the research object, in which, an optimum HPLC method was built to determine the content of the baicalin, being the index composition. Best program of water decoction of Radix Scutellariae was also studied with considering four factors, i. e. , comminution granularity, decoction time, amount of adding water and times of process. Experiments show that granularity is the determinative factor affecting lixiviation. The influencing degrees of four factors above on baicalin content decrease orderly. Optimum range of comminution granularity was obtained finally on the basis of building optimum program.

关 键 词：黄芩 黄芩苷 粉碎粒度 煎煮工艺 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]