豫医无毛小鼠无毛基因的比较生物学分析  

Analysis on comparative biology of hairless gene in Yuyi hairless mice

作　　者：章金涛[1] 王纯耀[1] 杜春燕[1] 

机构地区：[1]郑州大学实验动物中心,郑州450052

出　　处：《郑州大学学报（医学版）》2008年第3期450-452,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目39970119;河南省自然科学基金资助项目511040500

摘　　要：目的:分析豫医无毛小鼠与其他物种间无毛基因组成的差异。方法:采用RT-PCR方法对豫医无毛小鼠无毛基因的cDNA序列进行克隆测序,并借助生物信息学分析技术对小鼠、大鼠、猴和人的无毛基因及其所编码的氨基酸序列进行比较生物学分析。结果:获得了豫医无毛小鼠无毛基因的全长4 014 bp的cDNA序列(GenBank登录号:AY547390)。豫医无毛小鼠与国内外报道的2种小鼠、大鼠、猴、人等5种动物的核苷酸同源性分别为99.9%、99.7%、94.3%、81.7%、81.8%,氨基酸同源性分别是99.8%、99.7%、94.5%、79.8%、80.0%。结论:无毛基因是一个高度保守基因,不同物种间具有高度同源性。Aim： To study the difference of hairless gene between Yuyi hairless ,nice and others species. Methods： RT-PCR technique was developed to clone and analyze the sequence of cDNA in hairless gene from Yuyi hairless ,nice, and to analyze comparatively biologic character of amino acid sequence encoding hairless gene in different species. Results： A full-length cDNA of Yuyi hairless ,nice was 4 014 bp, which has been accepted by GenBank （ Accession Number： AY547390）. The identities of nucleotide of hairless gene were 99.9% , 99.7% , 94.3 % , 81.7 % , 81.8% by homologous comparison among Yuyi hairless ,nice and other species, and those of amino acid sequences were 99.8% ,99.7% ,94.5% , 79.8% ,80.0% ,respectively. Conclusion： The results suggest that the hairless gene is a highly-conserved gene and has higher homology among different mammalian taxons.

关 键 词：无毛小鼠 无毛基因 克隆 比较生物学 

分 类 号：Q953[生物学—动物学]