不同肾病患者血清自体荧光光谱分析  

Auto-fluorescence spectra analysis of different nephropathy patients′ serum

作　　者：阎素清[1] 贾小燕[2] 任丽克[2] 柴保臣[2] 

机构地区：[1]郑州大学公共卫生学院卫生化学教研室,郑州450001 [2]郑州大学公共卫生学院营养与食品卫生学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2008年第3期522-524,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关基金资助项目00524410098

摘　　要：目的：比较不同肾病患者的特征血清自体荧光光谱，为肾病的光谱学诊断提供理论依据。方法：应用F-4500荧光分光光度计，激发光波长为301nm，测定84例正常人，32例肾炎患者，28例肾衰患者及20例肾癌患者的血清在200～800nm发射光范围内的荧光光谱，对其荧光光谱的峰位置、峰高及峰面积进行了分析。结果：与正常对照组比较，肾炎、肾衰组在335nm和445nm附近的荧光峰峰位发生红移，与肾炎、肾衰组比较，肾癌组335nm附近的荧光峰峰位发生紫移；与正常对照组相比，肾病各组在302nm、335nm、445nm、515nm、650nm附近荧光光谱峰高、峰面积均有升高或降低，差异有统计学意义（P〈0．05）。结论：测定肾病患者血清自体荧光光谱在肾病的诊断中有一定的应用价值。Aim：To compare the auto-fluorescent spectrum of different nephropathy patients＇ serum, so as to provide theoretical foundation for spectroscopy diagnosis of nephropathy. Methods： Using F-4500 spectrophoto fluorometer, wavelength was 301 nin and emission wavelength ranged from 200 nm to 800 nm to measure fluorescence spectra of 84 normal controls ,32 nephritis patients,28 renal failure patients ,20 renal cancer patients. The peak heights ,peak positions, and peak areas of fluorescence spectra were analyzed. Results：Compared with normal control, the peak position occurred red shift near 335 nin and 445 nin in patients with nephritis and renal cancer; compared with the patients with nephritis and renal failure, the peak position occurred purple shift near 335 nm in patients with renal cancer. Compared with normal control, the peak height and the peak area decreased or increased significantly near 302 nm,335 nin, 445 nm, 515 nm, and 650 nm in different nephropathy patients （P 〈 0.05 ）. Conclusion： Measuring auto-fluorescence spectra of nephropathy patients＇ serum can provide some valuable evidence for diagnosis of nephropathy.

关 键 词：血清 荧光光谱 肾病 

分 类 号：O433[机械工程—光学工程]