磷酸酰肌醇-3激酶抑制剂对小鼠气道炎症影响的实验研究  

Phosphatidylinositol 3-kinase inhibitor reduces respiratory tract inflammation in a murine model of Asthma

作　　者：喻宁芬[1] 喻宁芳[2] 邓礼[1] 于力[1] 翁志媛[1] 陈英[1] 肖雪[1] 张又祥[1] 李书华[3] 

机构地区：[1]广东省广州市第一人民医院儿科,510180 [2]广东省广州市第一人民医院检验科,510180 [3]广州医学院病理教研室

出　　处：《中国医药》2008年第7期388-389,共2页China Medicine

基　　金：广东省科技计划资助项目（73092）;广州市医药卫生科技资助项目（2007-YB-026）

摘　　要：目的观察磷酸酰肌醇-3激酶（P13K）抑制剂LY294002对卵白蛋白诱导哮喘小鼠气道炎症的影响。方法Balb／c小鼠30只，采用随机数字表法分为3组，每组10只：LY294002处理组、卵白蛋白组、正常对照组。通过卵白蛋白多次腹腔注射致敏和反复雾化激发，建立哮喘小鼠模型。末次抗原激发后48h收集支气管肺泡灌洗液和骨髓标本，计数细胞总数和嗜酸性粒细胞，并行肺组织学检查。结果与卵白蛋白组比较，LY294002处理组支气管肺泡灌洗液中细胞总数及嗜酸性粒细胞绝对数明显减少（P〈0．05）：肺组织中细支气管、血管周围嗜酸性粒细胞浸润得到控制，气道黏液分泌减少。结论P13K特异性抑制剂LY294002对卵白蛋白致敏的哮喘小鼠气道炎症具有抑制作用。Objective To investigate the effect of phosphoinostitide 3-kinase inhibitor on bronchial inflammation in a murine model of asthma. Methods Thirty BALB/c mice were randomly divided into 3 groups （ 10 each group）： PI3K inhibitor （LY294002）-treated group, ovalbumin （OVA） group and normal control group. BALB/c mice were sensitized and challenged with OVA. Cells of bronchoalveolar larvage fluid （BALF） and bone marrow were counted and classified 2 days post last OVA sensitized. Lung tissues were sectioned to hematoxylin-eosin and periodic acid schiff staining. Results LY294002 treatment reduced OVA-induced airway infiltration of inflammatory cells, eosinophils in BALF, inflammatory infiltration and eosinophils numbers around airway, blood vessels or in lung histological sections. Conclusion PI3K inhibitor is effective in treating OVA-induced asthma by attenuating airway inflammation.

关 键 词：哮喘 气道炎症 磷酸酰肌醇-3激酶 

分 类 号：R562.2[医药卫生—呼吸系统]