漆酶/HBT介质系统对靛蓝染料及废水脱色的初步研究  

Preliminary study on decolorization of indigo dye and waste water with laccase/HBT mediator system

作　　者：刘晓波[1] 闫世梁[1] 李宗伟[1] 李培睿[1] 李宗义[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,河南郑州450052

出　　处：《环境污染与防治》2008年第6期27-30,共4页Environmental Pollution & Control

基　　金：国家重点基础研究发展计划资助项目(No2004CB719604)

摘　　要：以云芝(Trametes versicolor)1126发酵所得漆酶粗酶液与1-羟基苯并三唑(HBT)组成的漆酶/HBT介质系统对靛蓝染料进行脱色实验,分别考察了温度、转速、pH和HBT与漆酶的加入量等条件对靛蓝染料脱色的影响。最终确定的优化脱色条件为:温度60℃,转速200r/min,pH4.5,100mL靛蓝染料"溶液"中粗酶液和HBT溶液分别加入2mL。以上述脱色条件对靛蓝印染废水进行脱色实验,反应80min,脱色率可达90.1%。The degradation of the indigo dye with a laccase/HBT mediator system was studied. The mediator system was composed of laccase from Trarnetes versicolor 1126 and 1-hydroxy benzotriazole （HBT）. The effects of temperature, rotational speed,initial pH, the dosage of enzyme and HBT on the decolorization of indigo dye were also tested. The optimal condition was obtained as follows： temperature at 60 ℃, rotational speed of 200 r/min, pH at 4.5,crude laccase of 2 mL/100 mL, and HBT solution of 2 mL/100 mL. The decolorization experiments of indigo wastewater were conducted under the optimal condition. The results showed that the decolorization rate reached 90.1G in 80 min.

关 键 词：漆酶/HBT介质系统 靛蓝 脱色 

分 类 号：X703[环境科学与工程—环境工程]