HPLC法测定苦参中苦参碱的含量  

DETERMINATION OF MATRINE IN RADIX SOPHORAE FLAVESCENTIS BY HPLC

作　　者：田吉[1] 何兵[1] 雷素娟[2] 李春红[1] 艾洪兵[1] 

机构地区：[1]泸州医学院:药学院药物研究所,四川泸州646000 [2]泸州医学院:附属医院肝胆外科,四川泸州646000

出　　处：《泸州医学院学报》2008年第3期258-261,共4页Journal of Luzhou Medical College

基　　金：四川省教育厅青年基金项目(2000-B12)

摘　　要：目的:建立苦参中苦参碱含量的测定方法。方法:采用HPLC法,色谱柱为Kromasil C18柱(250mm×4.6mm,5μm),流动相为甲醇-水-二乙胺(55∶45∶0.02),检测波长208nm,柱温40℃;流速1.0m·lmin-1;进样量:10!l。结果:苦参碱在0.05～1.25μg范围内具有良好的线性关系(r=0.9999),平均回收率97.62%,RSD为1.15%(n=6)。结论:本方法灵敏、准确、简便快速,可用于苦参药材的质量控制。Objective ：To establish a method for the determination of matrine in Radix Sophorae Flavescentis. Methods ： A Kromasil C18 column （250mm×4.6mm, 5 μm）with mobile phase consisting of methanol-water-diethylamine （55：45：0.02） was adopted,and the detecting wavelength was set at 208 nm. The column temperature was 40℃. The flow rate was 1.0 ml·min^-1,and the sample size was 10μl.Results：The calibration curve showed good linearity over the range of 0.05～1.25μg （r=0.9999）. The average recovery was 97.62 % with RSD 1.15%（n=6）. Conclusion：The method is sensitive,accurate,rapid,and is suitable for the quality control of Radix Sophorae Flavescentis.

关 键 词：苦参 苦参碱 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]