镉和铜离子诱导棕尾别麻蝇幼虫脂质过氧化丙二醛动态变化分析  

Analysis on dynamic change of malondialdehyde induced by Cd^(2+) and Cu^(2+) in Boettcherisca peregrina larvae

作　　者：吴国星[1] 高熹[1] 叶恭银[2] 胡萃[2] 程家安[2] 

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]浙江大学昆虫科学研究所,杭州310029

出　　处：《环境昆虫学报》2008年第2期115-119,共5页Journal of Environmental Entomology

基　　金：国家自然科学基金重点项目(30230070)

摘　　要：采用饲料含毒法,测定了棕尾别麻蝇Boettcherisca peregrine1日龄幼虫连续取食含不同浓度Cd2+或Cu2+的人工饲料,体内丙二醛含量的变化过程。实验结果表明:二种重金属离子能诱导昆虫的脂质过氧化反应,并且存在浓度依赖效应和时间依赖效应。在0~1600μg/g饲料浓度范围内,丙二醛(malondialdehyde,MDA)含量与幼虫取食Cd2+和Cu2+的浓度呈线性关系,所求回归方程分别为y=0.2789+0.0002x和y=0.3141+0.0002x。当处理浓度为800μg/g时,在0~120h时间内,MDA含量与幼虫取食Cd2+和Cu2+的时间呈线性关系,回归方程分别为y=0.3041+0.0042x和y=0.3251+0.0029x。The dynamical variations of malondialdehyde in Boettcherisca peregrine larvae were studied af- ter one-day-old instar larvae were fed on the artificial diet containing various concentration of Cd^2＋ or Cu^2 ＋. The results showed that increasing of LPO was induced in B. peregrine larvae by Cd^2 ＋ or Cu^2 ＋ indose-dependent and time-dependent effects. When the exposure concentrations were in the range of 0 - 1600 μg/g diet, the linear relationship was found between the malondialdehyde （MDA, LPO product and a marker of oxidative stress） concentrations and Cd^2＋ or Cu^2＋ concentrations in the artificial diets. The regression equations for Cd^2＋ and Cu^2＋ were as follows： y =0. 2789 ＋0. 0002 x and y =0. 3141 ＋ 0. 0002 x. There was a linear relationship between the MDA concentration and feeding time, during the 120 h at the level of dietary Cd^2＋ or Cu^2＋ （800 μg/g） . The regression equations were y =0. 3041 ＋ 0. 0042x and y = 0. 3251 ＋ 0. 0029 x, respectively.

关 键 词：棕尾别麻蝇 CD^2+ CU^2+ 丙二醛 

分 类 号：Q966[生物学—昆虫学]