两头尖总苷的抗肿瘤活性研究  

Anti-tumor Activity of Crude Saponin from Anemone raddeana Regel

作　　者：王明奎[1] 丁立生[1] 吴凤锷[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2008年第3期378-382,共5页Chinese Journal of Applied and Environmental Biology

摘　　要：采用水解和溶剂萃取的方法从中药两头尖中提取制备了待测总皂苷,并研究了总皂苷的体外和体内的抑瘤活性.总皂苷的主要成分是银莲花素A,占总苷的20%.体外试验选用4种人癌细胞KB、HCT-8、MCF-7/WT和MCF-7/ADR,用MTT法测定总皂苷的抑制率,测得IC50分别为7.68、18.52、17.34和19.43μg/mL,并且对表阿霉素无交叉耐药性.体内试验选取小鼠肉瘤S180、小鼠肝癌H22和艾氏腹水癌EAC测试总皂苷的抑瘤率,采用灌胃的方式,总苷在1g/kg时对S180、H22和EAC的平均抑瘤率分别可达到68.1%、62.5%和69.3%.对总苷的急性毒性也进行了测定.通过灌胃给药和腹腔注射,测得LD50分别为5.7g/kg和106mg/kg.上述结果说明,采用本方法制备的两头尖总苷具有较好的抑制肿瘤生长的活性,是一个有潜力的抗癌药物.The crude saponin from Anemone raddeana Regel was prepared by hydrolysis and solvent extraction. The major component was raddeanin A which accounted for 20% of crude saponin. Its anti-tumor activity in-vitro and in vivo was investigated, The inhabitation on KB, HCT-8, MCF-7WT and MCF-7/ADR was measured with MTT in-vitro, and the ICso was 7.68, 18,52, 17.34 and 19.43 μg/mL respectively, with no cross-resistance to epirubicin. The inhabitation on tumor S180, liver cancer H22 and EAC mice was measured in vivo. By intragastric infusion at 1 g/kg, the inhabitation on S180, H22 and EAC was 68.1%, 62.5% and 69.3%, respectively. The acute toxicity of the crude saponin was also measured. The LD50 of intragastric infusion and intra-peritoneal administration were 5.7 g/kg and 106 mg/kg. Therefore, the crude saponin prepared in this way had good inhibitory activity on tumors in-vitro and in vivo, indicating the saponin is possibly a potential anti-cancer medicine.

关 键 词：两头尖 总苷 抑瘤活性 急性毒性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]